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searching for Cas9 288 found (427 total)

alternate case: cas9

Gene knockout (2,524 words) [view diff] exact match in snippet view article find links to article

through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs. One of the main advantages of gene knockouts is that they allow

Horticulture (3,624 words) [view diff] exact match in snippet view article find links to article

such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), are also improving the nutrition, taste and yield of crops. There are many

Glabrousness (530 words) [view diff] exact match in snippet view article find links to article

marker for plant research to improve gene editing methods such as CRISPR/Cas9. In varying degrees most mammals have some skin areas without natural hair

Germline mutation (3,080 words) [view diff] exact match in snippet view article find links to article

protein Cas9 to break the DNA backbones at specific target sequences. This system has shown a higher specificity than TALENs or ZFNs due to the Cas9 protein

Site-directed mutagenesis (2,973 words) [view diff] exact match in snippet view article find links to article

to site-directed mutagenesis. Since 2013, the development of the CRISPR/Cas9 technology, based on a prokaryotic viral defense system, has also allowed

Epigenome editing (3,122 words) [view diff] exact match in snippet view article find links to article

proteins, Transcription Activator-Like Effectors (TALEs) and nuclease deficient Cas9 fusions (CRISPR). Comparing genome-wide epigenetic maps with gene expression

CRISPR/Cas tools (1,247 words) [view diff] exact match in snippet view article find links to article

(January 2017). "Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA". Journal

DNA-binding domain (2,399 words) [view diff] exact match in snippet view article find links to article

complementarity to target DNA sites. Cas9 can be used as a customizable RNA-guided DNA-binding platform. Domain Cas9 can be functionalized with regulatory

He Jiankui (6,082 words) [view diff] exact match in snippet view article find links to article

evolution, including that of CRISPR, He learned gene-editing techniques (CRISPR/Cas9) as a postdoctoral researcher at Stanford University in California. He Jiankui

Mutagenesis (molecular biology technique) (2,884 words) [view diff] exact match in snippet view article

more specific changes can be made. Since 2013, development of the CRISPR/Cas9 technology, based on a prokaryotic viral defense system, has allowed for

Anopheles (4,122 words) [view diff] exact match in snippet view article find links to article

resistance and switch to other means if resistance is detected. In 2016, a CRISPR-Cas9 gene drive system was proposed to eradicate Anopheles gambiae, by deleting

Andrea Crisanti (scientist) (1,106 words) [view diff] exact match in snippet view article

malaria incidence. In 2018, Crisanti and colleagues demonstrated that CRISPR/Cas9 can be programmed to attack a conserved region of the sex determination gene

IRAK3 (708 words) [view diff] exact match in snippet view article find links to article

encoded by the IRAK3 gene. Using in vivo liposome-mediated delivery of CRISPR/Cas9 plasmid expressing IRAK3 gRNA, IRAK3 was shown to be responsible for endotoxin-induced

Candida albicans (9,894 words) [view diff] exact match in snippet view article find links to article

knowledge on essential genes can be used to discover novel antifungals. CRISPR/Cas9 has been adapted to be used in C. albicans. Several studies have been performed

Saccharomyces boulardii (2,012 words) [view diff] exact match in snippet view article find links to article

at 37 °C (98.6 °F). In addition, the popular genome-editing tool CRISPR-Cas9 was proven to be effective in S. boulardii. Boulard first isolated this yeast

Small nucleolar RNA (3,791 words) [view diff] exact match in snippet view article find links to article

that direct RNA editing in trypanosomes or the guide RNAs (gRNAs) used by Cas9 for CRISPR gene editing. After transcription, nascent rRNA molecules (termed

Trichome (2,860 words) [view diff] exact match in snippet view article find links to article

marker for plant research to improve gene editing methods such as CRISPR/Cas9. Trichomes also serve as models for cell differentiation as well as pattern

De-extinction (5,864 words) [view diff] exact match in snippet view article find links to article

advancing with the help of the CRISPR/Cas systems, particularly CRISPR/Cas9. The CRISPR/Cas9 system was originally discovered as part of the bacterial immune

Christine Merlin (2,477 words) [view diff] exact match in snippet view article find links to article

In 2016, Merlin and colleagues demonstrated that both TALENs and CRISPR/Cas9 technologies could be utilized in a similar manner to create highly efficient

Trichome (2,860 words) [view diff] exact match in snippet view article find links to article

marker for plant research to improve gene editing methods such as CRISPR/Cas9. Trichomes also serve as models for cell differentiation as well as pattern

Missense mRNA (1,009 words) [view diff] exact match in snippet view article find links to article

single-base substitutions, novel technologies that co-inject gRNA and hCas9 mRNA of the CRISPR/Cas9 system, in conjunction with single-strand oligodeoxynucleotide

Institute of Chemical Biology and Fundamental Medicine (269 words) [view diff] exact match in snippet view article find links to article

coronavirus and determines whether it belongs to the omicron strain. The CRISPR/Cas9 genome editing was discovered by molecular biologists Jennifer Doudna (United

Trans-activating crRNA (405 words) [view diff] exact match in snippet view article find links to article

crRNA/tracrRNA hybrid. This hybrid acts as a guide for the endonuclease Cas9, which cleaves the invading nucleic acid. CRISPR Deltcheva E, Chylinski K

Functional genomics (3,756 words) [view diff] exact match in snippet view article find links to article

of disrupted genes can be assigned based on observed phenotypes. CRISPR-Cas9 has been used to delete genes in a multiplexed manner in cell-lines. Quantifying

Transcription activator-like effector nuclease (3,147 words) [view diff] exact match in snippet view article find links to article

editing with engineered nucleases. Alongside zinc finger nucleases and CRISPR/Cas9, TALEN is a prominent tool in the field of genome editing. TAL effectors

Gene knock-in (1,568 words) [view diff] exact match in snippet view article find links to article

inserted with a CRISPR/Cas9 system, which allows for much more accurate and successful gene insertions. The speed of CRISPR/Cas9-mediated gene knock-in

Intellia Therapeutics (1,607 words) [view diff] exact match in snippet view article find links to article

specific to the disease-causing gene and messenger RNA that encodes the Cas9 enzyme, which carries out the precision editing. Intellia also has a number

Dharmacon (554 words) [view diff] exact match in snippet view article find links to article

CRISPR-Cas9 IP licensed to GE Dharmacon™ Edit-R™ Gene Engineering System | The Scientist Magazine® GE Healthcare Dharmacon Advances CRISPR Cas9 Gene Engineering

Ectocarpus (2,574 words) [view diff] exact match in snippet view article find links to article

both forward and reverse genetic methodologies, the latter based on CRISPR-Cas9. Brown algae are heterokonts, a group that also includes diatoms and oomycetes

Molecular genetics (3,842 words) [view diff] exact match in snippet view article find links to article

Crispr/Cas9, which was adapted from the genome immune defense that is naturally occurring in bacteria. This technique relies on the protein Cas9 which

SKBR3 (363 words) [view diff] exact match in snippet view article find links to article

cancers. The cell line has also been examined for applications in CRISPR/Cas9 gene editing, antibody resistance in transfections, and HER2-based cancer

Rodolphe Barrangou (465 words) [view diff] exact match in snippet view article find links to article

Advisory Board of Intellia Therapeutics. His research focuses on CRISPR-Cas9 in bacteria. In 2017, Barrangou was named Editor-in-Chief of The CRISPR Journal

Catgrip (478 words) [view diff] exact match in snippet view article find links to article

one, two or three metal ions. An example is given by residues 586-590 of Cas9 endonuclease (pdb code 4oge) where the mainchain CO groups from residues

Kevin M. Esvelt (1,194 words) [view diff] no match in snippet view article find links to article

Kevin Michael Esvelt is an American biologist. He is currently an assistant professor at the MIT Media Lab and leads the Sculpting Evolution group. After

Niveen Khashab (1,679 words) [view diff] exact match in snippet view article find links to article

DNA, and proteins. She focuses on the encapsulation and delivery of CRISPR-Cas9 to enhance targeted gene editing. With more than ten years of professional

List of awards and honors received by Jennifer Doudna (1,739 words) [view diff] exact match in snippet view article find links to article

to biochemistry and genetics, and is most famous for her work on CRISPR-Cas9 genome editing technology. Doudna and Emmanuelle Charpentier were awarded

Lei Stanley Qi (556 words) [view diff] exact match in snippet view article find links to article

Qi led the development of the first catalytically dead Cas9 lacking endonuclease activity (dCas9), which is the basis for CRISPR interference (CRISPRi)

15-Cis-phytoene desaturase (898 words) [view diff] exact match in snippet view article find links to article

successful genome editing in fruit such as apples, grapes or bananas using CRISPR/Cas9 systems. In rice, the natural PDS was supplemented by its bacterial homolog

Senolytic (1,986 words) [view diff] exact match in snippet view article find links to article

Narimani M, Sharifi M, Jalili A (2019-11-27). "Knockout Of BIRC5 Gene By CRISPR/Cas9 Induces Apoptosis And Inhibits Cell Proliferation In Leukemic Cell Lines

Gene therapy (17,769 words) [view diff] exact match in snippet view article find links to article

moratorium on inheritable human genome edits, including those related to CRISPR-Cas9 technologies but that basic research including embryo gene editing should

Thermoascus (458 words) [view diff] exact match in snippet view article find links to article

Agrobacterium tumefaciens-mediated transformation protocol and a CRISPR-Cas9 gene editing system, which have been used to overexpress genes that correspond

Zinc-finger nuclease (4,657 words) [view diff] exact match in snippet view article find links to article

used to precisely alter the genomes of higher organisms. Alongside CRISPR/Cas9 and TALEN, ZFN is a prominent tool in the field of genome editing. The DNA-binding

CRISPR Therapeutics (918 words) [view diff] exact match in snippet view article find links to article

a strategic research collaboration in 2015 focused on the use of CRISPR/Cas9 to discover and develop potential new treatments aimed at the underlying

HAP1 cells (919 words) [view diff] exact match in snippet view article find links to article

the Chromosome 15 region by the endonuclease Cas9, which led to the elimination of the fragment. The Cas9 was programmed by short guide RNAs (gRNAs) to

Duchenne muscular dystrophy (6,468 words) [view diff] exact match in snippet view article find links to article

Duchenne muscular dystrophy (DMD). Researchers used a technique called CRISPR/Cas9-mediated genome editing, which can precisely remove a mutation in the dystrophin

Fibroblast growth factor 8 (2,175 words) [view diff] exact match in snippet view article find links to article

been documented to play a role in oralmaxillogacial diseases and CRISPR-cas9 gene targeting on FGF8 may be key in treating these diseases. Cleft lip and/or

Myostatin-related muscle hypertrophy (708 words) [view diff] exact match in snippet view article find links to article

S, et al. (December 2015). "Generation of gene-target dogs using CRISPR/Cas9 system". Journal of Molecular Cell Biology. 7 (6): 580–3. doi:10.1093/jmcb/mjv061

Yuet Wai Kan (3,604 words) [view diff] exact match in snippet view article find links to article

correction of β-thalassemia mutations in patient-specific iPSCs using CRISPR/Cas9 and piggyBac". Genome Research. 24 (9): 1526–1533. doi:10.1101/gr.173427

Francisco Mojica (1,955 words) [view diff] exact match in snippet view article find links to article

were later developed into the first widespread genome editing tool, CRISPR-Cas9. Mojica was born in Elche, Spain, on 5 October 1963. He attended Los Andes

NIMPLY gate (128 words) [view diff] exact match in snippet view article find links to article

Fussenegger, Martin; Bojar, Daniel; Kim, Hyojin (9 April 2019). "A CRISPR/Cas9-based central processing unit to program complex logic computation in human

Penicillium chrysogenum (1,409 words) [view diff] exact match in snippet view article find links to article

the penicillin gene cluster. Similar to other filamentous fungi, CRISPR/Cas9-mediated genome editing techniques are available for editing the genome of

Francisco Mojica (1,955 words) [view diff] exact match in snippet view article find links to article

were later developed into the first widespread genome editing tool, CRISPR-Cas9. Mojica was born in Elche, Spain, on 5 October 1963. He attended Los Andes

Fibrolamellar hepatocellular carcinoma (2,189 words) [view diff] exact match in snippet view article find links to article

deletion, and formation of the DNAJB1::PRKACA chimeric gene, using CRISPR/Cas9 in the livers of mice. That the actual formation of the DNAJB1::PRKACA was

Fluorine-18 (872 words) [view diff] exact match in snippet view article find links to article

PET and fluorescence imaging of genome modified cells, e.g. cancer, CRISPR/Cas9, or CAR T-cells, in an entire mouse. The dual-modality small molecule targeting

TBX5 (gene) (2,467 words) [view diff] exact match in snippet view article

similar to their frontlimb wings. A gene "knockout" model for TBX5 by CRISPR/Cas9 genome editing has been created. This homozygous TBX5 knockout human embryonic

Genetically modified insect (2,396 words) [view diff] exact match in snippet view article find links to article

Dimitris; Gribble, Matthew; Baker, Dean; Marois, Eric (2015-12-07). "A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector

HacDC (492 words) [view diff] exact match in snippet view article find links to article

Gentle Hacker's Literary Salon RepRap 3D printer [1] HacDC Spaceblimp CRISPR-Cas9 bacterial gene editing project. Scanning electron microscope refurbishment

Molecular Infection Medicine Sweden (397 words) [view diff] exact match in snippet view article find links to article

the driving force behind the discovery of the mechanisms behind the CRISPR-Cas9 system. MIMS web page – www.mims.umu.se UCMR web page – www.ucmr.umu.se "Remote

C11orf53 (473 words) [view diff] exact match in snippet view article find links to article

acidic conditions, which are typical of the tumor microenvironment. CRISPR-Cas9 inactivation of C11orf53 in an acute myeloid leukemia cell line made the

Kiran Musunuru (1,216 words) [view diff] exact match in snippet view article find links to article

and is a leading expert in genome-editing techniques, particularly CRISPR-Cas9. His lab was the first to develop an efficient technique to genetically modify

Xenopus (5,805 words) [view diff] exact match in snippet view article find links to article

J, Thomsen GH, Grainger RM (December 2013). "Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis". Genesis. 51 (12): 835–843

Wild-type transthyretin amyloid (1,240 words) [view diff] exact match in snippet view article find links to article

study demonstrated that NTLA-2001, a therapeutic agent based on the CRISPR-Cas9 system, induces targeted knockout of the transthyretin protein. Because of

Neisseria meningitidis (4,640 words) [view diff] exact match in snippet view article find links to article

Components from Neisseria meningitidis are being harnessed in biotechnology. Its Cas9 enzyme is a useful tool in CRISPR gene editing because the enzyme is small

Transformation (genetics) (6,769 words) [view diff] case mismatch in snippet view article

mushroom strain edited with CRISPR/CAS9 to prevent fruit body browning causing a broad discussion about placing CRISPR/CAS9-edited crops on the market. Physical

Sickle cell disease (15,226 words) [view diff] exact match in snippet view article find links to article

original unmodified cells. CRISPR-Cas9: Genome Editing for Sickle Cell Anemia Researchers are investigating the use of CRISPR-Cas9, a highly specific and potent

Yield10 Bioscience (996 words) [view diff] exact match in snippet view article find links to article

acknowledging that its camelina line has had a gene disrupted using CRISPR/Cas9 gene editing technology, resulting in the desired phenotype. In 2019, Yield10

Micropeptide (3,894 words) [view diff] no match in snippet view article find links to article

Micropeptides (also referred to as microproteins) are polypeptides with a length of less than 100-150 amino acids that are encoded by short open reading

Jung-Min Lee (865 words) [view diff] exact match in snippet view article find links to article

targeted therapy. [1] Her research has expanded to include the use of CRISPR-Cas9 mediated therapeutic editing of genes involved in disease causing such as

Microbial cell factory (1,858 words) [view diff] case mismatch in snippet view article find links to article

cost. The CRISPR/CAS9 has been utilized to enhance MCFs to produce yeast, bacteria, and E.coli. When optimizing yeast, CRISPR/CAS9 promoting S.pyogenes

David M. Sabatini (1,955 words) [view diff] exact match in snippet view article find links to article

human cells, most notably through the use of RNA interference and the CRISPR-Cas9 system. 2009 Paul Marks Prize for Cancer Research 2014 NAS Award in Molecular

Gene knockdown (1,611 words) [view diff] exact match in snippet view article find links to article

Braff JL, Moosburner M, Yaung SJ, Church GM (November 2013). "Orthogonal Cas9 proteins for RNA-guided gene regulation and editing" (PDF). Nature Methods

Selfish genetic element (10,236 words) [view diff] exact match in snippet view article find links to article

countervailing selection, they are expected to go to fixation in a population. CRISPR-Cas9 technology allows the artificial construction of homing endonuclease systems

MELK (977 words) [view diff] exact match in snippet view article find links to article

PMID 16266996. Lin A, Giuliano CJ, Sayles NM, Sheltzer JM (March 2017). "CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going

Somatic cell (1,488 words) [view diff] exact match in snippet view article find links to article

Gupta R, Zhang M, et al. (March 2017). "Somatic genome editing with CRISPR/Cas9 generates and corrects a metabolic disease". Scientific Reports. 7: 44624

Excision BioTherapeutics (280 words) [view diff] exact match in snippet view article find links to article

developing gene therapies against HIV infection. The company has a single, CRISPR–Cas9 based therapy, EBT-101, under investigation. Initial investigation into the

HIV/AIDS research (5,105 words) [view diff] exact match in snippet view article find links to article

PMID 24020860. Ophinni Y, Inoue M, Kotaki T, Kameoka M (May 2018). "CRISPR/Cas9 system targeting regulatory genes of HIV-1 inhibits viral replication in

Eradication of suffering (1,469 words) [view diff] exact match in snippet view article find links to article

; Gould, Fred (2017-09-08). "Evaluating strategies for reversing CRISPR-Cas9 gene drives". Scientific Reports. 7 (1): 11038. Bibcode:2017NatSR...711038V

PARP inhibitor (2,052 words) [view diff] exact match in snippet view article find links to article

Aleksandrov R, et al. (May 2018). "Genome-wide and high-density CRISPR-Cas9 screens identify point mutations in PARP1 causing PARP inhibitor resistance"

Bioassay (1,436 words) [view diff] exact match in snippet view article find links to article

"Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library". Scientific Reports. 9 (1): 3780. doi:10.1038/s41598-019-40381-4

Wolf Prize in Medicine (216 words) [view diff] exact match in snippet view article find links to article

Charpentier France for deciphering and repurposing the bacterial CRISPR/Cas9 immune system for genome editing. Jennifer Doudna United States for revealing

New England Biolabs (2,210 words) [view diff] exact match in snippet view article find links to article

agreement with ERS Genomics Limited that gave NEB rights to sell CRISPR/Cas9 tools and reagents, used for gene editing. The NEBuilder HiFi DNA Assembly

Macular degeneration (9,795 words) [view diff] exact match in snippet view article find links to article

testing to guide clinical management is not currently recommended. CRISPR-Cas9 genome editing may be used to treat wet age-related macular degeneration

David and Lucile Packard Foundation (730 words) [view diff] exact match in snippet view article find links to article

Fellows' work has contributed to breakthroughs like the creation of the CRISPR-Cas9 gene-editing technique, the discovery of soft tissues in dinosaur fossils

Horizontal Environmental Genetic Alteration Agents (1,447 words) [view diff] exact match in snippet view article find links to article

2015). "Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System". Molecular Plant. 8 (8): 1288–91. doi:10.1016/j.molp.2015.02.011

Exagamglogene autotemcel (1,433 words) [view diff] exact match in snippet view article find links to article

autotemcel is the first cell-based gene therapy treatment utilizing CRISPR/Cas9 gene editing technology to be approved by the US Food and Drug Administration

Embryo (3,281 words) [view diff] exact match in snippet view article find links to article

attempted - see He Jiankui affair) genetic editing of human embryos via CRISPR-Cas9 as a potential avenue for preventing disease; however, this has been met

David Kirsch (459 words) [view diff] exact match in snippet view article find links to article

Gersbach, CA, Dodd, RD, and Kirsch, DG. "Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma." Nature

Killifish (2,320 words) [view diff] exact match in snippet view article find links to article

was achieved in Nothobranchius furzeri using a draft genome and the CRISPR/Cas9 system. By targeting multiple genes, including telomerase, the killifish

Protein & Cell (242 words) [view diff] exact match in snippet view article find links to article

Songyang, Zhou; Ma, Wenbin; Zhou, Canquan; Huang, Junjiu (2015). "CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes". Protein & Cell. 6

Qinling panda (944 words) [view diff] exact match in snippet view article find links to article

scientists underwent an experiment testing mice where they utilized CRISPR-Cas9, a gene-editing tool. The team was able to manipulate the sequence of the

Hyperphosphatasia with mental retardation syndrome (593 words) [view diff] exact match in snippet view article find links to article

Stumpf A, Pitsch J, Tsortouktzidis D, et al. (January 2021). "A CRISPR-Cas9-engineered mouse model for GPI-anchor deficiency mirrors human phenotypes

Theodore Roosevelt High School (Iowa) (998 words) [view diff] exact match in snippet view article

MIT neurobiologist, co-inventor of optogenetics, developer of the CRISPR/Cas9 gene editing method, winner of the Perl-UNC Neuroscience Prize Des Moines

Vienna Biocenter (418 words) [view diff] exact match in snippet view article find links to article

Charpentier and Jennifer Doudna for their groundbreaking discoveries on the CRISPR/Cas9 system. Emmanuelle Charpentier was a principal investigator at the Max Perutz

Genetic engineering (14,201 words) [view diff] exact match in snippet view article find links to article

Jennifer Doudna and Emmanuelle Charpentier collaborated to develop the CRISPR/Cas9 system, a technique which can be used to easily and specifically alter the

Timeline of biology and organic chemistry (2,810 words) [view diff] exact match in snippet view article find links to article

the most popular high-throughput sequencing system. 2012 – Use of CRISPR-Cas9 as a DNA-editing biotechnology tool. Timeline of medicine and medical technology

Modifications (genetics) (2,627 words) [view diff] exact match in snippet view article

distribution to patients with diabetes. Fast-paced developments in the CRISPR-Cas9 gene editing technology has increased both the concerns and relevance of

Overlapping gene (5,334 words) [view diff] exact match in snippet view article find links to article

variety of reverse genetics techniques, such as CRISPR-Cas9 and catalytically dead Cas9 (dCas9) disruption. Attempts at proof-by-synthesis are also performed

Opisthorchiasis (1,948 words) [view diff] exact match in snippet view article find links to article

31 (6): 1156–63. doi:10.4269/ajtmh.1982.31.1156. PMID 6983303. "CRISPR/Cas9 shown to limit impact of certain parasitic diseases". www.bionity.com. Retrieved

David R. Liu (2,497 words) [view diff] exact match in snippet view article find links to article

the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions". Nature Biotechnology. 35 (4): 371–376. doi:10

NAS Award in Chemical Sciences (1,259 words) [view diff] exact match in snippet view article find links to article

the technology for efficient site-specific genome engineering using CRISPR/Cas9 nucleases. Armand Paul Alivisatos (2017) For making fundamental contributions

Retinitis pigmentosa (6,401 words) [view diff] exact match in snippet view article find links to article

study by Bakondi et al. at Cedars-Sinai Medical Center showed that CRISPR/Cas9 can be used to treat rats with the autosomal dominant form of retinitis pigmentosa

NAS Award in Molecular Biology (1,740 words) [view diff] exact match in snippet view article find links to article

systems, groundbreaking work that catalyzed the manipulation of the CRISPR-Cas9 pathway for genome engineering. 2018 Howard Y. Chang for the discovery of

Genetically modified organism (24,527 words) [view diff] exact match in snippet view article find links to article

nucleases, transcription activator-like effector nucleases (TALENs), and the Cas9-guideRNA system (adapted from CRISPR). TALEN and CRISPR are the two most

Sarcospan (1,579 words) [view diff] exact match in snippet view article find links to article

preclinical studies. Additionally, gene editing technologies, such as CRISPR-Cas9, have been used to correct sarcospan mutations in muscle cells, offering

Gene cassette (1,316 words) [view diff] exact match in snippet view article find links to article

the uptake and expression of the new genetic elements. The usage of CRISPR/Cas9 systems has shown success in inserting genes into eukaryotic genomes. While

Essential gene (8,521 words) [view diff] exact match in snippet view article find links to article

lead to erroneous annotations (here: false negatives). Comparison of CRISPR/cas9 and RNAi screens. Screens to identify essential genes in the human chronic

Diamondback moth (4,400 words) [view diff] exact match in snippet view article find links to article

precise research to identify suitable genetic targets. Using the CRISPR/Cas9 system as a targeted gene to identify the abdominal segment, thus removing

Management of HIV/AIDS (15,824 words) [view diff] exact match in snippet view article find links to article

pluripotent stem cells that have been CCR5 disrupted through the CRISPR/Cas9 gene editing system. The main obstacle to complete elimination of HIV infection

Nucleotide (3,299 words) [view diff] exact match in snippet view article find links to article

essential for the proper function of gene-editing technologies such as CRISPR-Cas9. Nucleotide (abbreviated "nt") is a common unit of length for single-stranded

Bacillus cereus (6,438 words) [view diff] exact match in snippet view article find links to article

When point mutations were introduced into the plcR gene using the CRISPR/Cas9 system, it was observed that the mutated bacteria lost their hemolytic and

TGF beta receptor 1 (1,618 words) [view diff] exact match in snippet view article find links to article

Shukla, Sanjeev; Chande, Ajit (June 2022). "Improved loss-of-function CRISPR-Cas9 genome editing in human cells concomitant with inhibition of TGF-β signaling"

MODY 2 (684 words) [view diff] exact match in snippet view article find links to article

"Genetic deletion of a short fragment of glucokinase in rabbit by CRISPR/Cas9 leading to hyperglycemia and other typical features seen in MODY-2". Cellular

Arabidopsis thaliana (8,632 words) [view diff] exact match in snippet view article find links to article

marker for plant research to improve gene editing methods such as CRISPR/Cas9. In 2005, scientists at Purdue University proposed that A. thaliana possessed

Max Planck Institute for Infection Biology (999 words) [view diff] exact match in snippet view article find links to article

nobelprize.org. Retrieved 7 October 2020. "Emmanuelle Charpentier, CRISPR-Cas9, Max Planck Institute for Infection Biology". Max Planck Unit for the Science

Karmella Haynes (1,656 words) [view diff] exact match in snippet view article find links to article

pericentric reporter (94 citations) The impact of chromatin dynamics on Cas9-mediated genome editing in human cells (82 citations) Engineering bacteria

Research Science Institute (1,296 words) [view diff] exact match in snippet view article find links to article

Institute and MIT neurobiologist, optogenetics pioneer, developer of the CRISPR/Cas9 gene editing method, and "35 Innovators Under 35" laureate Feng Zhang attended

Familial amyloid polyneuropathy (1,486 words) [view diff] exact match in snippet view article find links to article

Retrieved 11 August 2018. Gillmore, Julian D. (August 5, 2021). "CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis". The New England Journal

Victoria Gray (319 words) [view diff] exact match in snippet view article find links to article

the first patient to be treated for sickle-cell disease using the CRISPR-Cas9 gene-editing technology. Thanks to her gene-edited cells, Gray has been cured

Monoclonal antibody (4,794 words) [view diff] exact match in snippet view article find links to article

of recombinant monoclonal antibodies involves repertoire cloning, CRISPR/Cas9, or phage display/yeast display technologies. Recombinant antibody engineering

Maneesha S. Inamdar (1,387 words) [view diff] case mismatch in snippet view article find links to article

knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39". Stem Cell Research. 16 (2):

Development, Growth & Differentiation (298 words) [view diff] exact match in snippet view article find links to article

T. & Okumura, M. 63 488- 500 2021 Efficient visual screening of CRISPR/Cas9 genome editing in the nematode Pristionchus pacificus Diaz-Cuadros, Margarete

Maneesha S. Inamdar (1,387 words) [view diff] case mismatch in snippet view article find links to article

knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39". Stem Cell Research. 16 (2):

Development, Growth & Differentiation (298 words) [view diff] exact match in snippet view article find links to article

T. & Okumura, M. 63 488- 500 2021 Efficient visual screening of CRISPR/Cas9 genome editing in the nematode Pristionchus pacificus Diaz-Cuadros, Margarete

MAGESTIC (600 words) [view diff] exact match in snippet view article find links to article

specific variants to be tracked in pools, similar to how Genome-wide CRISPR-Cas9 knockout screens work, only MAGESTIC is more versatile as it allows for not

Organoid (9,321 words) [view diff] exact match in snippet view article find links to article

intestinal cystic fibrosis organoid phenotype could be repaired by CRISPR-Cas9 gene editing in 2013. Follow-up studies by Dekkers et al. in 2016 revealed

Kang Zhang (2,573 words) [view diff] exact match in snippet view article find links to article

Zhang K, Belmonte JC (December 2016). "In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration". Nature. 540 (7631):

Alexander Varshavsky (2,631 words) [view diff] exact match in snippet view article find links to article

single-subunit split proteins, such as split-GFP, split lactamase, split Cas9 CRISPR nuclease, and many other split protein sensors and effectors. (viii)

Nissim Benvenisty (1,641 words) [view diff] exact match in snippet view article find links to article

2018). "Defining essential genes for human pluripotent stem cells by CRISPR-Cas9 screening in haploid cells". Nature Cell Biology. 20 (5): 610–619. doi:10

Choroideremia (3,087 words) [view diff] exact match in snippet view article find links to article

safety issues related to the transplanted tissue." A 2015 study used CRISPR/Cas9 to repair mutations in patient-derived induced pluripotent stem cells that

LRP2 (2,606 words) [view diff] exact match in snippet view article find links to article

"Cubilin-, megalin-, and Dab2-dependent transcription revealed by CRISPR/Cas9 knockout in kidney proximal tubule cells". American Journal of Physiology

GENCODE (2,626 words) [view diff] exact match in snippet view article find links to article

2018, one of the latest additions to the GENCODE project was the CRISPR/Cas9 track on human and model organism assemblies. CRISPR is a genome editing

Nonsense mutation (2,870 words) [view diff] exact match in snippet view article find links to article

initiating chain termination, upon encountering premature stop codons. CRISPR-Cas9 based single nucleotide substitutions have been used to generate amino acid

Medical microbiology (3,979 words) [view diff] exact match in snippet view article find links to article

Bacteria can also eventually become resistant, through systems like CRISPR/Cas9 system. Many clinical trials have been promising though, showing that it

Human Frontier Science Program (1,467 words) [view diff] exact match in snippet view article find links to article

University of California, Berkeley, USA), "for their seminal work on the CRISPR-Cas9 system". 2017 David Julius (University of California, San Francisco, USA)

Hilo High School (1,360 words) [view diff] exact match in snippet view article find links to article

Dad Jennifer Doudna (1981), American biochemist, developer of the CRISPR-Cas9 genome editing method, 2020 Nobel laureate Lorraine Inouye, Hawaii County

Molecular biology (5,527 words) [view diff] exact match in snippet view article find links to article

nations and increase accessibility to individual researchers. Likewise, CRISPR-Cas9 gene editing experiments can now be conceived and implemented by individuals

SPATA16 (461 words) [view diff] exact match in snippet view article find links to article

Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models". International Journal of Molecular Sciences. 18 (10):

Har Gobind Khorana (2,839 words) [view diff] exact match in snippet view article find links to article

referred to his research while advancing genome editing with the CRISPR/Cas9 system. After years of work, he was the first in the world to complete the

Emerging technologies (4,138 words) [view diff] exact match in snippet view article find links to article

Justin S; Handgretinger, Rupert; Mezger, Markus (May 20, 2020). "CRISPR/Cas9 technology: towards a new generation of improved CAR-T cells for anticancer

5-Hydroxymethylcytosine (1,744 words) [view diff] exact match in snippet view article find links to article

June 2015). "Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9". mBio. 6 (3): e00648. doi:10.1128/mBio.00648-15. PMC 4471564. PMID 26081634

Transcriptome instability (364 words) [view diff] exact match in snippet view article find links to article

splicing and genomic instability after induction of DNMT3A mutations by CRISPR/Cas9 gene editing". Blood Cells, Molecules and Diseases. 69: 10–22. doi:10.1016/j

Tang Prize (1,059 words) [view diff] exact match in snippet view article find links to article

Biopharmaceutical Science Emmanuelle Charpentier France "for the development of CRISPR/Cas9 as a breakthrough genome editing platform that promises to revolutionize

Cynthia Sharma (1,326 words) [view diff] exact match in snippet view article find links to article

crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9. Science 372: 941-948. doi:10.1126/science.abe7106 Eisenbart, S. K., M. Alzheimer

Adenoviridae (5,557 words) [view diff] exact match in snippet view article find links to article

all the viral genome.: 58 Adenovirus has been used for delivery of CRISPR/Cas9 gene editing systems, but high immune reactivity to viral infection has posed

KBM-7 cells (688 words) [view diff] exact match in snippet view article find links to article

Patrick; et al. (December 2014). "Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line". Genome Research. 24 (12): 2059–206

Kathy Niakan (626 words) [view diff] exact match in snippet view article find links to article

healthy embryo. In 2017 her lab published the first major study using CRISPR-Cas9 in human embryos in Nature, demonstrating that the transcription factor Oct4

Michel Sadelain (2,399 words) [view diff] exact match in snippet view article find links to article

Sadelain, Michel (2017–03). "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection" Nature. 543 (7643): 113–117. doi:10.1038/nature21405

Late endosomal/lysosomal adaptor, mapk and mtor activator 1 (542 words) [view diff] exact match in snippet view article find links to article

2017). "Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-coupled Affinity Purification/Mass Spectrometry Analysis Revealed a Novel

Jian-Kang Zhu (2,374 words) [view diff] exact match in snippet view article find links to article

Plant Biol. 6:441-445. Miki D, Zhang W, Zeng W, Feng Z, Zhu JK. 2018. CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation. Nature

HEK 293 cells (3,065 words) [view diff] case mismatch in snippet view article find links to article

equilibrative nucleoside transporter ENT1. The gene was knocked out using CRISPR-CAS9 and the cell line retains ENT2 expression. HEK 293 cells are straightforward

Zebrafish (11,535 words) [view diff] exact match in snippet view article find links to article

are well-established protocols for editing zebrafish genes using CRISPR-Cas9 and this tool has been used to generate genetically modified models. In 2008

Aquaculture of tilapia (2,795 words) [view diff] exact match in snippet view article find links to article

provides a biological lock mechanism. Gene editing is coming. Especially CRISPR-Cas9 can already be implemented and holds a lot of promise. We also expect for

Non-homologous end joining (3,679 words) [view diff] exact match in snippet view article find links to article

Viet Q. (May 2020). "Dual Supramolecular Nanoparticle Vectors Enable CRISPR/Cas9‐Mediated Knockin of Retinoschisin 1 Gene—A Potential Nonviral Therapeutic

Aspergillus oryzae (2,628 words) [view diff] exact match in snippet view article find links to article

complicated. However, scientists have recently started utilizing CRISPR/Cas9 in A. oryzae. This increased mutation rates in the genome which was not possible

CAR T cell (8,019 words) [view diff] exact match in snippet view article find links to article

to a partial deletion of the U3 region. The new gene editing tool CRISPR/Cas9 has recently been used instead of retroviral vectors to integrate the CAR

Fatty-acid amide hydrolase 1 (3,457 words) [view diff] exact match in snippet view article find links to article

using transcriptomic analyses of cell models, some created anew using CRISPR-Cas9, others obtained from the 2019 patient. The study confirms that FAAH-OUT

Nepidermin (1,174 words) [view diff] exact match in snippet view article find links to article

"Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library". Scientific Reports. 9 (1): 3780. Bibcode:2019NatSR...9.3780Q. doi:10

Japan Prize (631 words) [view diff] exact match in snippet view article find links to article

Interspaced Short Palindromic Repeats)-Cas and the creation of the CRISPR-Cas9 genome editing system, a truly revolutionary technique in genetic engineering

Medical genetics (5,257 words) [view diff] exact match in snippet view article find links to article

Zhou; Ma, Wenbin; Zhou, Canquan; Huang, Junjiu (18 April 2015). "CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes". Protein & Cell. 6

Cystic fibrosis (17,980 words) [view diff] exact match in snippet view article find links to article

unusable. There has been a functional repair in culture of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. Bacteriophage

Methanococcus maripaludis (2,983 words) [view diff] exact match in snippet view article find links to article

ribosome-binding sites using CRISPR/Cas9 technology. Large deletions in the DNA can also be facilitated by a CRISPR/Cas9 system specifically designed for

Diatom (15,271 words) [view diff] exact match in snippet view article find links to article

(16 February 2018). "An Expanded Plasmid-Based Genetic Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways in Phaeodactylum

Amyloidosis (5,432 words) [view diff] exact match in snippet view article find links to article

Taubel J, Kao J, Fontana M, Maitland ML, et al. (August 2021). "CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis". The New England Journal

Anatoly B. Kolomeisky (982 words) [view diff] exact match in snippet view article find links to article

Anatoly B. (2017). "Mechanism of Genome Interrogation: How CRISPR RNA-Guided Cas9 Proteins Locate Specific Targets on DNA". Biophysical Journal. 113 (7). Elsevier

Shoukhrat Mitalipov (904 words) [view diff] exact match in snippet view article find links to article

attempt at genetically correcting mutant human embryos, using the CRISPR/Cas9 gene modifying tool. Mitalipov and his team experimented upon a larger number

Taosheng Huang (1,438 words) [view diff] exact match in snippet view article find links to article

human diseases, and has used induced pluripotent stem cell models and CRISPR/Cas9-produced mouse models in his lab to explore the pathogenesis of mutations

James A. Wells (2,036 words) [view diff] exact match in snippet view article find links to article

(subtiligase and the SNIPer), E3 ligase substrates (the NEDDylator), a split-Cas9 for temporal editing, and allosteric inhibitors, split-kinases and new phosphospecific

Nannochloropsis (2,985 words) [view diff] exact match in snippet view article find links to article

percentages in Nannochloropsis gaditana through applications of the CRISPR-Cas9 reverse-genetics pipeline and nitrogen starving—improving partitioning of

Harvey Prize (200 words) [view diff] exact match in snippet view article find links to article

2018 Emmanuelle Charpentier France for leading the development of CRISPR-Cas9 technology, a breakthrough in genetic modification. Jennifer Doudna U.S.

Mosquito (9,075 words) [view diff] exact match in snippet view article find links to article

Machine. BBC. Kyrou, Kyros Kyrou; et al. (24 September 2018). "A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in

Capitella teleta (4,604 words) [view diff] exact match in snippet view article find links to article

of gene perturbation techniques such as Morpholino knockdown and CRISPR-Cas9 mutagenesis, and methods for living imaging such as mRNA injection. The development

Aedes aegypti (4,971 words) [view diff] exact match in snippet view article find links to article

no progeny. Male mosquitoes do not bite or spread disease. Using CRISPR/Cas9 based genome editing to engineer the genome of Aedes aegypti genes like ECFP

Genetically modified virus (3,843 words) [view diff] exact match in snippet view article find links to article

Zhang F (April 2015). "In vivo genome editing using Staphylococcus aureus Cas9". Nature. 520 (7546): 186–91. Bibcode:2015Natur.520..186R. doi:10.1038/nature14299

DNA repair (15,713 words) [view diff] exact match in snippet view article find links to article

clustered regularly interspaced short palindromic repeat (shortened to CRISPR-Cas9) was discovered in 2012. The new technology allows anyone with molecular

Magnetofection (2,407 words) [view diff] exact match in snippet view article find links to article

Xiaoyun (2020). "A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing". Theranostics. 10 (12): 5532–5549. doi:10.7150/thno

Kiana Aran (1,021 words) [view diff] exact match in snippet view article find links to article

et al. (March 25, 2019), Detection of unamplified target genes via CRISPR-Cas9 immobilized on a graphene field-effect transistor, vol. 3, pp. 427–437, doi:10

Viral vector (4,962 words) [view diff] exact match in snippet view article find links to article

encoding, for instance, complementary DNA, short hairpin RNA, and CRISPR/Cas9 for gene editing. Viral vectors can be used for cellular reprogramming, for

Vilnius University (9,821 words) [view diff] exact match in snippet view article find links to article

Šikšnys is recognized for his contributions to the development of CRISPR/Cas9 gene editing technology, often referred to as 'gene scissors'. He currently

Genetics (10,393 words) [view diff] exact match in snippet view article find links to article

Liang P, Xu Y, Zhang X, Ding C, Huang R, Zhang Z, et al. (May 2015). "CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes". Protein & Cell. 6

XL-413 (248 words) [view diff] exact match in snippet view article find links to article

Wyman SK, et al. (April 2020). "Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair". Nature Communications. 11 (1): 2109. Bibcode:2020NatCo

Selective organ targeting (2,174 words) [view diff] exact match in snippet view article find links to article

Siegwart, Daniel J. (2020-06-26). "Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing". Nature

Eugenics (10,855 words) [view diff] exact match in snippet view article find links to article

Alex; Hornblower, Breton; Robb, Brett; Tzertzinis, George (2014). "CRISPR/Cas9 and Targeted Genome Editing: A New Era in Molecular Biology". NEB Expressions

ALS (14,008 words) [view diff] exact match in snippet view article find links to article

human induced pluripotent stem cell (iPSC) technologies coupled with CRISPR/Cas9 gene-editing, and human post-mortem spinal cord tissue examination. Excitotoxicity

UCK2 (2,398 words) [view diff] exact match in snippet view article find links to article

Carette JE, Khosla C, Bassik MC (May 2016). "Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification". Nature Chemical Biology

James Wilson (scientist) (688 words) [view diff] exact match in snippet view article

Xu, Chenyu; Morizono, Hiroki (March 2016). "A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice". Nature

Preclinical imaging (6,058 words) [view diff] exact match in snippet view article find links to article

PET and fluorescence imaging of genome modified cells, e.g. cancer, CRISPR/Cas9, or CAR T-cells, in an entire mouse. The combining of these imaging modalities

Non-small-cell lung cancer (5,301 words) [view diff] exact match in snippet view article find links to article

December 2021). "Epigenetic targeting for lung cancer treatment via CRISPR/Cas9 technology". Advances in Cancer Biology - Metastasis. 3: 100012. doi:10.1016/j

Protein tag (2,677 words) [view diff] exact match in snippet view article find links to article

leads to a rapid knock-in of this tag with other proteins through CRISPR/Cas9 technology. Affinity purification Protein array TimeSTAMP protein labelling

Precision BioSciences (1,642 words) [view diff] exact match in snippet view article find links to article

off-targeting. An ARCUS nuclease is also much smaller in size than CRISPR spCas9. It can use either adeno-associated virus (AAV) vectors or lipid nanoparticles

Gene (12,720 words) [view diff] exact match in snippet view article find links to article

Scott DA, Zhang F (November 2013). "Genome engineering using the CRISPR-Cas9 system". Nature Protocols. 8 (11): 2281–2308. doi:10.1038/nprot.2013.143

Alternative splicing (7,867 words) [view diff] exact match in snippet view article find links to article

splicing and genomic instability after induction of DNMT3A mutations by CRISPR/Cas9 gene editing". Blood Cells, Molecules & Diseases. 69: 10–22. doi:10.1016/j

Beta thalassemia (4,880 words) [view diff] exact match in snippet view article find links to article

"Vertex and CRISPR Therapeutics Announce Authorization of the First CRISPR/Cas9 Gene-Edited Therapy, Casgevy (exagamglogene autotemcel), by the United Kingdom

Gene targeting (4,385 words) [view diff] exact match in snippet view article find links to article

Mara K, Maclot F, Guyon-Debast A, Charlot F, et al. (January 2017). "CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent

Behavioural genetics (7,969 words) [view diff] exact match in snippet view article find links to article

knockouts, floxing, gene knockdown, or genome editing using methods like CRISPR-Cas9. These techniques allow behavioural geneticists different levels of control

Mitochondrial calcium uniporter (817 words) [view diff] exact match in snippet view article find links to article

mitochondrial calcium uniporter complex resumes. Research using a CRISPR/Cas9 technique has found that MICU1 and MICU2 play other roles as well. They are

Amyotrophic lateral sclerosis research (2,286 words) [view diff] exact match in snippet view article find links to article

motor cells can be grown, and the gene expression is controlled. CRISPR/Cas9 technique can be used to knock-out/in genes that are related to ALS, and

Norbert Perrimon (1,411 words) [view diff] exact match in snippet view article find links to article

genes in various cell-based assays. With Ram Viswanatha, he developed CRISPR/Cas9 pooled screens in Drosophila cells to facilitate large-scale screen in Drosophila

Association of Biomolecular Resource Facilities (1,893 words) [view diff] exact match in snippet view article find links to article

Emmanuelle Charpentier and Jennifer Doudna for the development of CRISPR/Cas9 Genome Editing Technologies. 2015 John G. White and William Bradshaw Amos

Amyotrophic lateral sclerosis research (2,286 words) [view diff] exact match in snippet view article find links to article

motor cells can be grown, and the gene expression is controlled. CRISPR/Cas9 technique can be used to knock-out/in genes that are related to ALS, and

Experimental models of Alzheimer's disease (3,543 words) [view diff] exact match in snippet view article find links to article

form of the disease. These cells can also be purchased commercially. CRISPR-Cas9 technology can be used alongside iPSC cells to generate neurons carrying

Glutamate carboxypeptidase II (4,300 words) [view diff] exact match in snippet view article find links to article

PET and fluorescence imaging of genome modified cells, e.g. cancer, CRISPR/Cas9, or CAR T-cells, in an entire mouse. PSMA can also be used as a target for

Daniel Voytas (671 words) [view diff] exact match in snippet view article find links to article

finger nucleases (ZFNs), TAL effector nucleases (TALEN), and the CRISPR/Cas9 system, Dan has achieved targeted gene knockouts, replacements and insertions

University of Texas Southwestern Medical Center (4,571 words) [view diff] exact match in snippet view article find links to article

damage suffered following a heart attack. Olson's lab has also used CRISPR/Cas9 methods to cure Duchenne muscular dystrophy in mice as a proof of principle

Primary ciliary dyskinesia (4,008 words) [view diff] exact match in snippet view article find links to article

functional replacement of cilia involve antisense, gene editing via CRISPR-Cas9 and mRNA therapies. At present there have only been a handful of interventional

Genetically modified crops (16,857 words) [view diff] exact match in snippet view article find links to article

Bi H, Fromm M, Yang B, Weeks DP (November 2013). "Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum

Ehlers–Danlos syndrome (11,320 words) [view diff] exact match in snippet view article find links to article

2021). "A new mouse model of Ehlers–Danlos syndrome generated using CRISPR/Cas9-mediated genomic editing". Disease Models & Mechanisms. 14 (12): dmm048963

Aaron Traywick (1,442 words) [view diff] exact match in snippet view article find links to article

Futurism. January 15, 2017. Retrieved May 6, 2018. "The Power of Crispr Cas9 & How to Create a Clearer Truth w/Aaron Traywick - TOT Revolution". TOT Revolution

Boron (12,763 words) [view diff] exact match in snippet view article find links to article

PET and fluorescent imaging of genome modified cells, e.g. cancer, CRISPR/Cas9, or CAR T-cells, in an entire mouse. The dual-modality small molecule targeting

RACGAP1 (1,608 words) [view diff] exact match in snippet view article find links to article

biogenesis in breast cancer. Knocking out RACGAP1 in vitro using CRISPR/Cas9 leads to cytokinesis failure. RACGAP1 has been shown to interact with ECT2

Huntington's disease (14,357 words) [view diff] exact match in snippet view article find links to article

genome with the erroneous gene that causes HD, using tools such as CRISPR/Cas9. Another strategy to reduce the level of mutant huntingtin is to increase

Nuclear organization (4,188 words) [view diff] exact match in snippet view article find links to article

At the same time, progress in genome-editing techniques (such as CRISPR/Cas9, ZFNs, and TALENs) have made it easier to test the organizational function

Mitochondrial replacement therapy (4,671 words) [view diff] exact match in snippet view article find links to article

Genetic Disease". Wall Street Journal. Fogleman, Sarah (2016-09-20). "CRISPR/Cas9 and mitochondrial gene replacement therapy: promising techniques and ethical

Schistosomiasis (10,040 words) [view diff] exact match in snippet view article find links to article

e0006968. doi:10.1371/journal.pntd.0006968. PMC 6300301. PMID 30532268. "CRISPR/Cas9 shown to limit impact of certain parasitic diseases". www.bionity.com. Retrieved

Caenorhabditis elegans (10,541 words) [view diff] exact match in snippet view article find links to article

C. elegans adult with GFP coding sequence inserted into a histone-encoding gene by Cas9-triggered homologous recombination

Institute of Chemical Technology (6,380 words) [view diff] exact match in snippet view article find links to article

Chitosan-Coated PLGA Nanocarriers for Cellular Delivery of siRNA and CRISPR/Cas9 Complex". Journal of Pharmaceutical Innovation. 17: 180–193. doi:10.1007/s12247-020-09496-4

Glypican (2,445 words) [view diff] exact match in snippet view article find links to article

GPC2 as a therapeutic target in neuroblastoma. Silencing of GPC2 by CRISPR/Cas9 results in the inhibition of neuroblastoma tumor cell growth. GPC2 silencing

Novozymes Prize (435 words) [view diff] exact match in snippet view article find links to article

Retrieved 2024-04-05. "Emmanuelle Charpentier wins Novozomes Prize for CRISPR-Cas9". www.umu.se. Retrieved 2024-04-05. Vilnius University: Novozymes Prize won

Xenotransplantation (8,032 words) [view diff] exact match in snippet view article find links to article

have been engineered to inactivate all 62 PERVs in the genome using CRISPR Cas9 genome editing technology, and eliminated infection from the pig to human

Malaria (23,937 words) [view diff] exact match in snippet view article find links to article

Fazekas A, Macias VM, Bier E, James AA (December 2015). "Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquito

Mouse models of breast cancer metastasis (5,955 words) [view diff] exact match in snippet view article find links to article

associated with a cancer critical gene p53 which was determined via CRISPR-Cas9. The quantitative lineage-tracing strategies have proven to be successful

Umbraulva (1,658 words) [view diff] exact match in snippet view article find links to article

Je Hyeong (2021). "Improving lignocellulosic biofuel production by CRISPR/Cas9‐mediated lignin modification in barley". GCB Bioenergy. 13 (4): 742–752.

Genetically modified food (15,670 words) [view diff] exact match in snippet view article find links to article

nucleases, transcription activator-like effector nucleases (TALENs), and the Cas9-guideRNA system (adapted from CRISPR). TALEN and CRISPR are the two most

Schistosoma mansoni (6,881 words) [view diff] exact match in snippet view article find links to article

In 2019, Ittiprasert, Brindley and colleagues employed programmed CRISPR/Cas9 knockout of the gene encoding the T2 ribonuclease of the egg of Schistosoma

Kathy Feng-Yi Su (863 words) [view diff] exact match in snippet view article find links to article

Gowri; Supeinthiran, Ahiraa; Meier, Rudolf; Su, Kathy F.Y. (2018). "CRISPR/Cas9 deletions in a conserved exon of Distal-less generates gains and losses in

2-Fluoroadenine (683 words) [view diff] exact match in snippet view article find links to article

Taizo; Nagasato, Chikako; Cock, J. Mark (2021-07-10). "Targeted CRISPR-Cas9-based gene knockouts in the model brown alga Ectocarpus" (PDF). New Phytologist

Timeline of Monsanto (711 words) [view diff] exact match in snippet view article find links to article

billion dollars. 2016 Products Monsanto buys a license from Broad Institute of Harvard University and MIT to use the CRISPR/Cas9 gene-editing technology.

Jacques Pouysségur (1,388 words) [view diff] exact match in snippet view article find links to article

Numerous anti-cancer targets inactivated by Zinc Finger Nucleases and/or CRISPR-Cas9 (carbonic anhydrases CA9, CA12, CA2, bicarbonate carriers NBC, lactate/H+

STING-associated vasculopathy with onset in infancy (1,098 words) [view diff] exact match in snippet view article find links to article

generated the first mouse model of SAVI. Dr. Miner's research team used CRISPR/Cas9 genome editing to introduce a mutation into the mouse STING gene (STING1)

Evolution of the brain (7,768 words) [view diff] exact match in snippet view article find links to article

archaic gene variant NOVA1 present in Neanderthals and Denisovans via CRISPR-Cas9 shows that it has a major impact on neurodevelopment and that such genetic

Xiaoliang Sunney Xie (2,634 words) [view diff] exact match in snippet view article find links to article

Wensheng; Xie, X. Sunney (2017). "Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging". Cell Res. 27 (2): 298–301. doi:10.1038/cr.2017.9

Baltic Assembly Prize for Literature, the Arts and Science (333 words) [view diff] exact match in snippet view article find links to article

exceptional achievements in biomedical sciences – pioneering research in CRISPR-Cas9 Genome editing" 2022 Gustavs Strenga, Andris Levāns, Renāte Berga, and Laura

W. Mark Saltzman (1,294 words) [view diff] exact match in snippet view article find links to article

"Efficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9". Circulation Research. 117 (2): 121–8. doi:10.1161/CIRCRESAHA.117.306290

List of Clarivate Citation laureates in Chemistry (262 words) [view diff] exact match in snippet view article find links to article

Emmanuelle Charpentier (born 1968) France "for the development of the CRISPR-cas9 method for genome editing." Umeå University Max Planck Institute for Infection

Aviation biofuel (4,640 words) [view diff] exact match in snippet view article find links to article

(October 18, 2016). "Genome editing of Clostridium autoethanogenum using CRISPR/Cas9". Biotechnology for Biofuels. 9 (1): 219. doi:10.1186/s13068-016-0638-3.

W. Mark Saltzman (1,294 words) [view diff] exact match in snippet view article find links to article

"Efficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9". Circulation Research. 117 (2): 121–8. doi:10.1161/CIRCRESAHA.117.306290

SaPI (1,430 words) [view diff] exact match in snippet view article find links to article

addition, by cloning, of antibacterial genes including those encoding CRISPR/cas9 with spacers targeting conserved chromosomal genes. These cause a lethal

Monarch butterfly (20,836 words) [view diff] exact match in snippet view article find links to article

into the same gene in the fruit fly Drosophila melanogaster using CRISPR-Cas9 genome editing. These fruit flies-turned monarch flies were completely resistant

2015 in science (23,993 words) [view diff] exact match in snippet view article find links to article

moratorium on inheritable human genome edits, including those related to CRISPR-Cas9 technologies. 4 December The Earth Institute at Columbia University publishes

John P. Morrissey (biologist) (1,459 words) [view diff] exact match in snippet view article

Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid. FEMS Yeast Researc 18 (3), 2018; S. foy012. doi:10

List of genetically modified crops (2,055 words) [view diff] exact match in snippet view article find links to article

Ming; Gilbert, Brian; Ayliffe, Michael (2016-07-01). "Applications of CRISPR/Cas9 technology for targeted mutagenesis, gene replacement and stacking of genes

Tissue engineering (13,967 words) [view diff] exact match in snippet view article find links to article

Human iPS Cell Line Precisely Corrected by Genome Editing Using the CRISPR/Cas9 System". Stem Cell Reviews and Reports. 11 (5): 774–87. doi:10.1007/s12015-015-9600-1

LMNA-related congenital muscular dystrophy (586 words) [view diff] exact match in snippet view article find links to article

the disease could be found through some advanced therapies such as CRISPR/Cas9. Muscular dystrophy Carboni N, Politano L, Floris M, Mateddu A, Solla E,

DNA (cytosine-5)-methyltransferase 3A (3,924 words) [view diff] exact match in snippet view article

splicing and genomic instability after induction of DNMT3A mutations by CRISPR/Cas9 gene editing". Blood Cells, Molecules & Diseases. 69: 10–22. doi:10.1016/j

Reuven Agami (891 words) [view diff] exact match in snippet view article find links to article

Leeuwenhoekziekenhuis. With the development of Ribosome profiling and CRISPR-Cas9 technologies, he further studied transcriptional Enhancers and mRNA translation

Facioscapulohumeral muscular dystrophy (16,335 words) [view diff] exact match in snippet view article find links to article

alteration of genetic code, is being researched. One study tried to use CRISPR-Cas9 to knockout the polyadenylation signal in lab dish models, but was unable

Margaret Goodell (1,170 words) [view diff] exact match in snippet view article find links to article

Genome Editing of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9". Cell Reports. 17 (5): 1453–1461. doi:10.1016/j.celrep.2016.09.092. PMC 5087995

Microbubble (3,928 words) [view diff] exact match in snippet view article find links to article

Yi, Yuping; Bao, Shan (May 2019). "Ultrasound microbubble-mediated CRISPR/Cas9 knockout of C-erbB-2 in HEC-1A cells". Journal of International Medical Research

Single-cell sequencing (8,560 words) [view diff] exact match in snippet view article find links to article

Bar-Joseph Z (June 2020). "Single-cell lineage tracing by integrating CRISPR-Cas9 mutations with transcriptomic data". Nature Communications. 11 (1): 3055

Sheila Singh (1,512 words) [view diff] exact match in snippet view article find links to article

Preclinical development and evaluation of CAR-T cells targeting CD70. CRISPR-Cas9 genetic screening to identify targets that act to increase sensitivity of

Drosophila suzukii (4,348 words) [view diff] exact match in snippet view article find links to article

Patil, Anandrao A.; Lloyd, Alun L.; Scott, Maxwell J. (2023-06-20). "CRISPR/Cas9-based split homing gene drive targeting doublesex for population suppression

Cassava brown streak virus disease (2,278 words) [view diff] exact match in snippet view article find links to article

C.; Staskawicz, Brian J.; Bart, Rebecca S. (2018). "Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava

Endocrine disruptor (16,616 words) [view diff] exact match in snippet view article find links to article

(thousands of lines) of rodents. The most recent tool used to do this is CRISPR/Cas9 which allows this process to be done more efficiently. Genes may be manipulated

Health equity (18,056 words) [view diff] exact match in snippet view article find links to article

gene therapy treatments that use gene editing machinery known as CRISPR/Cas9 to alleviate the sickling of the red blood cells. This will greatly improve

Marine protists (10,542 words) [view diff] exact match in snippet view article find links to article

Amit Kumar; Sparstad, Torfinn; Bones, Atle M.; Winge, Per (2016). "A CRISPR/Cas9 system adapted for gene editing in marine algae". Scientific Reports. 6:

Martin Zenke (2,139 words) [view diff] exact match in snippet view article find links to article

RNA-Seq, ChIP-seq, ATAC-seq, chromosome conformation capture (4C) and CRISPR/Cas9 gene editing, and more recently in the human system using induced pluripotent

AI-10-49 (2,849 words) [view diff] exact match in snippet view article find links to article

Deletion of the RUNX1 binding site in these enhancers by genome editing (CRISPR/Cas9) reduced MYC transcript levels and the viability of inv(16) AML cells, indicating

RepSox (730 words) [view diff] exact match in snippet view article find links to article

Sanjeev; Chande, Ajit (8 March 2022). "Improved loss-of-function CRISPR-Cas9 genome editing in human cells concomitant with inhibition of TGF-β signaling"

Wendell Lim (2,189 words) [view diff] exact match in snippet view article find links to article

invented the CRISPRi system that used modular DNA targeting of inactive Cas9 to control the transcription of specific endogenous genes. He has also been

Halperin-Birk syndrome (1,457 words) [view diff] exact match in snippet view article find links to article

further contribute to the COP-II vesicle and cargo selection diversity. CRISPR/Cas9-mediated knockdown of the SEC31A gene in human SH-SY5Y neuroblastoma cells

BLESS (1,978 words) [view diff] exact match in snippet view article find links to article

be artificially induced using genome editing technologies such as CRISPR-Cas9 or TALEN. These technologies may lead to unintentional modifications of DNA

Vera Meyer (1,992 words) [view diff] exact match in snippet view article find links to article

synthetic biology and systems biology. Gene technology methods, such as CRISPR-Cas9 and the generation of huge omics data, are being used for predicting gene

S. Matthew Liao (3,525 words) [view diff] exact match in snippet view article find links to article

raised by advances in reproductive technology, such as the use of CRISPR-Cas9 for germline gene editing, ‘three-parent IVF’ or mitochondrial replacement

Live single-cell imaging (2,210 words) [view diff] exact match in snippet view article find links to article

Conner, David A.; Hu, Johnny H.; Seidman, Christine E. (2018-01-24). "CRISPR/Cas9 Mediated Fluorescent Tagging of Endogenous Proteins in Human Pluripotent

Major histocompatibility complex, class I-related (3,218 words) [view diff] exact match in snippet view article find links to article

Caillaud ME, Lloyd A, Attaf M, et al. (February 2020). "Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic

Pharmacoepigenetics (4,224 words) [view diff] exact match in snippet view article find links to article

assess potential synthetic lethal interactions is using siRNA and CRISPR-Cas9 to modify target genes. CRISPRi and CRISPRa technology allows researchers

Steven M. Reppert (4,234 words) [view diff] exact match in snippet view article find links to article

Christine (2016). "Genomic Access to Monarch Migration Using TALEN and CRISPR/Cas9-Mediated Targeted Mutagenesis". G3 (Bethesda). 6 (4): 905–15. doi:10.1534/g3

List of female Clarivate Citation laureates (295 words) [view diff] exact match in snippet view article find links to article

Emmanuelle Charpentier (born 1968) France "for the development of the CRISPR-cas9 method for genome editing." Umeå University Max Planck Institute for Infection

Tofazzal Islam (3,548 words) [view diff] exact match in snippet view article find links to article

Tofazza Islam. Scientific Reports (2018) 8:2504. 2. Application of CRISPR/Cas9 Genome Editing Technology for the Improvement of Crops Cultivated in Tropical

Robert Pollack (biologist) (6,807 words) [view diff] exact match in snippet view article

(26 April 2018). "Modern Prometheus: Editing the Human Genome with Crispr-Cas9". Cambridge University Press. doi:10.1017/9781108597104.004. "Robert Pollack"

2018 in science (22,287 words) [view diff] exact match in snippet view article find links to article

University and the Chinese Academy of Sciences report the use of CRISPR/Cas9 to develop a variety of rice producing 25-31% more grain than traditional

Clinical metagenomic sequencing (3,492 words) [view diff] exact match in snippet view article find links to article

mtRNA, globin mRNA) using sequence-specific RNA depletion kits. Also CRISPR-Cas9-based approaches can be performed to target and deplete human mitochrondrial

Mendel Lectures (857 words) [view diff] exact match in snippet view article find links to article

Charpentier Max Planck Institute for Infection Biology, Berlin, Germany "CRISPR-Cas9: a Bacterial Immune System Repurposed as a Transformative Genome Engineering

VPS35 (4,050 words) [view diff] exact match in snippet view article find links to article

modulate VPS35 using viral vectors or genome editing techniques like CRISPR/Cas9, however, given VPS35's ubiquitous role in many homeostatic processes, strict

Digital microfluidics (10,668 words) [view diff] exact match in snippet view article find links to article

error rates and improve reproducibility. An automated DMF system for CRISPR-Cas9 genome editing was described by Sinha et al, and was used to culture and

Moisés Expósito-Alonso (1,100 words) [view diff] exact match in snippet view article find links to article

combines large-scale common garden experiments and genome sequencing and CRISPR/Cas9 to study the consequence of gene edits in Arabidopsis. His research has been

Functional cloning (3,006 words) [view diff] exact match in snippet view article find links to article

Bangmei; Jin, Shunqian; Reiser, Michelle; Lockey, Richard F (2015). "CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning". BioTechniques

SARM1 (5,231 words) [view diff] exact match in snippet view article find links to article

R, Carty M, Roche F, Fergus C, Hokamp K, et al. (December 2021). "CRISPR/Cas9-mediated SARM1 knockout and epitope-tagged mice reveal that SARM1 does not

History of research on Arabidopsis thaliana (5,679 words) [view diff] exact match in snippet view article find links to article

genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9". Nature Biotechnology. 31 (8): 688–691. doi:10.1038/nbt.2654. ISSN 1087-0156

Murine respirovirus (31,229 words) [view diff] exact match in snippet view article find links to article

to process F0. Sendai virus based vector system that can deliver CRISPR/Cas9 for efficient gene editing was created. A set of different recombinant SeV

Ricardo Dolmetsch (1,623 words) [view diff] exact match in snippet view article find links to article

Hoffman, G.R.; Dolmetsch, R.; Kaykas, A. (July 2018). "p53 inhibits CRISPR-Cas9 engineering in human pluripotent stem cells". Nat Med. 24 (7): 939–946. doi:10

Ultrasound-triggered drug delivery using stimuli-responsive hydrogels (5,059 words) [view diff] exact match in snippet view article find links to article

therapy drugs used in hydrogel-based drug delivery systems include CRISPR/Cas9, siRNA, and other RNA-based drugs. In a gene therapy study, Han et al. proposed

Arabinogalactan protein (8,970 words) [view diff] exact match in snippet view article find links to article

Marco (2017-09-22). "Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata". PLOS ONE.

List of single cell omics methods (4,871 words) [view diff] exact match in snippet view article find links to article

"Simultaneous lineage tracing and cell-type identification using CRISPR-Cas9-induced genetic scars". Nature Biotechnology. 36 (5): 469–473. doi:10.1038/nbt

RNA-directed DNA methylation (14,860 words) [view diff] exact match in snippet view article find links to article

(February 2019). "Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems". Nature Communications. 10 (1): 729. Bibcode:2019NatCo..10

CSNK1D (14,738 words) [view diff] exact match in snippet view article find links to article

Gluenz E, Späth GF, Rachidi N (2017). "Leishmania donovani Using the CRISPR Cas9 Toolkit". BioMed Research International. 2017: 4635605. doi:10.1155/2017/4635605

Hemoglobin D (3,748 words) [view diff] exact match in snippet view article find links to article

"Vertex and CRISPR Therapeutics Announce Authorization of the First CRISPR/Cas9 Gene-Edited Therapy, Casgevy (exagamglogene autotemcel), by the United Kingdom

April–June 2021 in science (11,867 words) [view diff] exact match in snippet view article find links to article

gene editing in which a – lipid nanoparticle formulated – CRISPR (with mCas9) gene editing therapeutic is injected in vivo into bloodstream of humans

July–September 2020 in science (19,713 words) [view diff] exact match in snippet view article find links to article

deleting gene drives introduced into populations in the wild with CRISPR-Cas9 gene editing. The paper's senior author cautions that the two neutralizing