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searching for DNA construct 15 found (27 total)

Gene gun (2,144 words) [view diff] exact match in snippet view article find links to article

involves the integration of a functional fragment of DNA—known as a DNA construct—into target cells. A gene construct is a DNA cassette containing all

Enhancer trap (240 words) [view diff] exact match in snippet view article find links to article

interest (which takes about a year when starting without the appropriate DNA construct), one transgenic fly is simply mated (crossed) with another transgenic

NdeI (348 words) [view diff] exact match in snippet view article find links to article

jelly fish Aequorea victoria. NdeI is useful in generating heterologous DNA construct because it contains the start codon ATG. It therefore can be used in

Sebelipase alfa (481 words) [view diff] exact match in snippet view article find links to article

Veterinary Medicine (CVM) approved the application for a recombinant DNA construct in genetically engineered chicken to produce rhLAL in their egg whites

H19 (gene) (6,242 words) [view diff] exact match in snippet view article

also contains a CpG site. In vitro methylation of this CpG site on a DNA construct strongly inhibited transcription of the H19 gene. In cell lines derived

AquAdvantage salmon (2,325 words) [view diff] exact match in snippet view article find links to article

instead of only during spring and summer. The stability of the new DNA construct was tested, revealing no additional mutational effects during insertion

Knockout moss (717 words) [view diff] no match in snippet view article find links to article

International Moss Stock Center. For altering moss genes in a targeted way, the DNA-construct needs to be incubated together with moss protoplasts and with polyethylene

Recombinant DNA (3,893 words) [view diff] exact match in snippet view article find links to article

the host organism, the foreign DNA contained within the recombinant DNA construct may or may not be expressed. That is, the DNA may simply be replicated

Fluorescent tag (2,775 words) [view diff] exact match in snippet view article find links to article

utilized depending on the nature of the target. In enzymatic labeling, a DNA construct is first formed, using a gene and the DNA of a fluorescent protein.

Molecular cloning (4,016 words) [view diff] exact match in snippet view article find links to article

to examine a number of different clones to be sure that the desired DNA construct is obtained. This may be accomplished through a very wide range of experimental

Physcomitrella patens (2,426 words) [view diff] exact match in snippet view article find links to article

strand are engineered to be identical to this specific gene locus. The DNA construct is then incubated with moss protoplasts in the presence of polyethylene

Viral vector (5,043 words) [view diff] exact match in snippet view article find links to article

To produce lentiviruses with the gene of interest as the lentiviral DNA construct, first transfect cells with a packaging plasmid and the envelope vector

Gene doping (3,911 words) [view diff] exact match in snippet view article find links to article

designed with either polymorphism, or for endurance athletes, some DNA construct that interfered with expression like a small interfering RNA. Myostatin

DNA-directed RNA interference (2,402 words) [view diff] exact match in snippet view article find links to article

medical intervention. Figure 2 illustrates the most common type of DDRNAI DNA construct, designed to express a shRNA. This figure consists of a promoter sequence

Artificial gene synthesis (7,073 words) [view diff] exact match in snippet view article find links to article

fusion linkers were also designed to allow for full read-through of the DNA construct. These fusion linkers code for a 15 amino acid glycine and serine polypeptide