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SDS-PAGE
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SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which isCreatinase (373 words) [view diff] exact match in snippet view article find links to article
enzyme was shown to be made up of two subunit monomers via SDS-polyacrylamide gel electrophoresis. The molecular weights of these subunits was estimated toSB buffer (308 words) [view diff] exact match in snippet view article find links to article
SB buffer is a buffer solution used in agarose and polyacrylamide gel electrophoresis for the separation of nucleic acids such as DNA and RNA. "SB" isIsoelectric point (2,871 words) [view diff] exact match in snippet view article find links to article
proteins. Isoelectric focusing is also the first step in 2-D gel polyacrylamide gel electrophoresis. In biomolecules, proteins can be separated by ion exchangeEastern blot (2,088 words) [view diff] exact match in snippet view article find links to article
most use phosphoprotein blotted from sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel on to a polyvinylidene fluoride or nitrocelluloseOligoclonal band (2,556 words) [view diff] exact match in snippet view article find links to article
fluids, also known as "SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis)/Coomassie blue staining", and (b) the combination of isoelectricUlrich K. Laemmli (454 words) [view diff] exact match in snippet view article find links to article
SDS polyacrylamide gel electrophoresis". MIT Faculty Newsletter. Massachusetts Institute of Technology. Retrieved 9 November 2023. "SDS-Polyacrylamide GelPhosphodiesterase (1,660 words) [view diff] exact match in snippet view article find links to article
subtypes) of phosphodiesterase were isolated from rat brain using polyacrylamide gel electrophoresis in the early 1970s by Weiss and coworkers, and were soon afterwardGlycophorin (273 words) [view diff] exact match in snippet view article find links to article
severe malaria. After separation of red cell membranes by SDS-polyacrylamide gel electrophoresis and staining with periodic acid-Schiff staining (PAS), fourElectroelution (299 words) [view diff] case mismatch in snippet view article find links to article
PMID 20834225. Kastenholz, B (2004). "Preparative Native Continuous Polyacrylamide Gel Electrophoresis (PNC‐PAGE): An Efficient Method for Isolating Cadmium CofactorsBromophenol blue (489 words) [view diff] exact match in snippet view article find links to article
to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Since bromophenol blue carries a slight negative charge atMammaglobin-B (603 words) [view diff] exact match in snippet view article find links to article
"Establishment of the human reflex tear two-dimensional polyacrylamide gel electrophoresis reference map: new proteins of potential diagnostic value"CD5L (508 words) [view diff] exact match in snippet view article find links to article
Schifferli JA, Hochstrasser DF, et al. (1994). "Two-dimensional polyacrylamide gel electrophoresis analysis of cryoglobulins and identification of an IgM-associatedTricine (309 words) [view diff] exact match in snippet view article find links to article
Schaegger, H., and von Jagow, G., "Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100Margaret Jope (452 words) [view diff] exact match in snippet view article find links to article
Shell VI: C Terminal end groups and sodium dodecyl sulphate-polyacrylamide gel electrophoresis: molecular constitution and structure of the protein", CompMary Osborn (1,610 words) [view diff] exact match in snippet view article find links to article
determination of the molecular weight of a protein via SDS polyacrylamide gel electrophoresis, published in 1969 in Journal of Biological Chemistry. TheyImmobilized pH gradient (602 words) [view diff] exact match in snippet view article find links to article
"Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in theProtein purification (3,910 words) [view diff] exact match in snippet view article find links to article
exclusion chromatography or by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) analysis. Proteins are often purified by using 2D-PAGE and40S ribosomal protein S3a (1,029 words) [view diff] exact match in snippet view article find links to article
copurifying with the CD4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: comparison with affinity-tag basedTrehalase (1,512 words) [view diff] exact match in snippet view article find links to article
molecular mass of 160 kDa, while in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) it showed a mass of 80 kDa. This hydrolase enzymeProtein mass spectrometry (3,815 words) [view diff] exact match in snippet view article find links to article
its isoelectric point (pI) and the second-dimension is SDS-polyacrylamide gel electrophoresis (SDS-PAGE). This dimension separates the protein accordingNeuroproteomics (2,577 words) [view diff] exact match in snippet view article find links to article
conditions. Proteins are commonly separated using two-dimensional polyacrylamide gel electrophoresis (2D PAGE). For this technique, proteins are run across anPolyacrylamide (1,616 words) [view diff] exact match in snippet view article find links to article
Raymond and Weintraub independently published on the use of polyacrylamide gel electrophoresis to separate charged molecules. The technique is widely acceptedMargot Forde (585 words) [view diff] exact match in snippet view article find links to article
"Grass cultivar identification by sodium dodecylsulphate polyacrylamide gel electrophoresis". New Zealand Journal of Agricultural Research. 29 (2): 193–206Sarah Young (immunologist) (398 words) [view diff] exact match in snippet view article
analysis of melanoma‐derived exosomes by two‐dimensional polyacrylamide gel electrophoresis and mass spectrometry." Proteomics 4, no. 12 (2004): 4019–4031Top-down proteomics (1,363 words) [view diff] exact match in snippet view article find links to article
quantitation. Top-down proteomics, when accompanied with polyacrylamide gel electrophoresis, can help to complement the bottom-up proteomic approach.Shotgun proteomics (1,660 words) [view diff] exact match in snippet view article find links to article
technologies to separate complex mixtures. In 1975, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was described by O’Farrell and Klose with the abilityNorthern blot (2,120 words) [view diff] exact match in snippet view article find links to article
observe the quality and quantity of RNA before blotting. Polyacrylamide gel electrophoresis with urea can also be used in RNA separation but it is mostPDE5 inhibitor (1,721 words) [view diff] exact match in snippet view article find links to article
adenosine-3',5'-monophosphate phosphodiesterase in rat cerebellum by polyacrylamide gel electrophoresis". Biochimica et Biophysica Acta. 284 (1): 220–6. doi:10Phosphodiesterase inhibitor (2,348 words) [view diff] exact match in snippet view article find links to article
3',5'-monophosphate phosphodiesterase in rat cerebellum by polyacrylamide gel electrophoresis". Biochim. Biophys. Acta. 284 (1): 220–226. doi:10.1016/0005-2744(72)90060-5Donald Crothers (1,045 words) [view diff] exact match in snippet view article find links to article
"Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis". Nucleic Acids Research. 9 (23): 6505–25. doi:10.1093/nar/9Carbonyl sulfide hydrolase (1,371 words) [view diff] exact match in snippet view article find links to article
ICP-MS to deduce the structure. Using sodium dodecyl sulfate–polyacrylamide gel electrophoresis, a subunit molecular mass of 27 kDa was found. After testingCGMP-specific phosphodiesterase type 5 (1,824 words) [view diff] exact match in snippet view article find links to article
adenosine-3′,5′-monophosphate phosphodiesterase in rat cerebellum by polyacrylamide gel electrophoresis". Biochimica et Biophysica Acta (BBA) - Enzymology. 284 (1):DNA footprinting (2,156 words) [view diff] exact match in snippet view article find links to article
from the cleavage agent. Run both samples side by side on a polyacrylamide gel electrophoresis. The portion of DNA template without protein will be cut atDNA-binding protein (2,563 words) [view diff] exact match in snippet view article find links to article
"Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis". Nucleic Acids Res. 9 (23): 6505–6525. doi:10.1093/nar/9Phosphoproteomics (1,541 words) [view diff] exact match in snippet view article find links to article
radioactive labeling with 32P-labeled ATP followed by SDS polyacrylamide gel electrophoresis or thin layer chromatography. These traditional methods areBand 3 anion transport protein (1,266 words) [view diff] exact match in snippet view article find links to article
was discovered following SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) of erythrocyte cell membrane. The large 'third' band on theVDAC2 (2,653 words) [view diff] exact match in snippet view article find links to article
copurifying with the CD4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: comparison with affinity-tag basedGeorge B. Johnson (1,312 words) [view diff] exact match in snippet view article find links to article
1979, Genetics 92: 357-360 Increasing the resolution of polyacrylamide gel electrophoresis by varying the degree of gel cross-linking, 1979, BiochemicalP4HB (2,221 words) [view diff] exact match in snippet view article find links to article
human colon carcinoma proteins separated by two-dimensional polyacrylamide gel electrophoresis". Electrophoresis. 11 (10): 883–91. doi:10.1002/elps.1150111019Coomassie brilliant blue (2,571 words) [view diff] exact match in snippet view article find links to article
can be used to separate proteins or protein complexes using polyacrylamide gel electrophoresis under non-denaturing conditions in a technique called blueGlycophorin C (3,060 words) [view diff] exact match in snippet view article find links to article
falciparum. After separation of red cell membranes by SDS-polyacrylamide gel electrophoresis and staining with periodic acid-Schiff staining (PAS) fourPRKAR2A (2,093 words) [view diff] exact match in snippet view article find links to article
of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine RIIs". The Journal of BiologicalWestern blot (8,047 words) [view diff] exact match in snippet view article find links to article
certain protein on the blot membrane. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is generally used for the denaturing electrophoreticUNSW Faculty of Science (1,560 words) [view diff] exact match in snippet view article find links to article
complexes. The resulting complexes can be further analysed by polyacrylamide gel electrophoresis and mass spectrometry. This will help identify the constituentARHGDIB (2,405 words) [view diff] exact match in snippet view article find links to article
Leavitt J (1990). "Sequence analysis of proteins separated by polyacrylamide gel electrophoresis: towards an integrated protein database". ElectrophoresisCryoglobulinemia (2,996 words) [view diff] exact match in snippet view article find links to article
Schifferli JA, Hochstrasser DF, et al. (1994). "Two-dimensional polyacrylamide gel electrophoresis analysis of cryoglobulins and identification of an IgM-associatedMargaret Sheil (2,151 words) [view diff] exact match in snippet view article find links to article
copurifying with the CD4/lck complex using one-dimensional polyacrylamide gel electrophoresis and mass spectrometry: Comparison with affinity-tag basedDenaturation (biochemistry) (3,056 words) [view diff] case mismatch in snippet view article
doi:10.1016/0006-3002(61)91013-7. PMID 13767022. "Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA". Electrophoresis. National Diagnostics. 15 AugustEukaryotic ribosome (3,977 words) [view diff] exact match in snippet view article find links to article
characterization of proteins separated by two-dimensional polyacrylamide gel electrophoresis". J Biol Chem. 270 (11): 6000–6005. doi:10.1074/jbc.270.11Lesley Joy Rogers (2,129 words) [view diff] exact match in snippet view article find links to article
"Separation of human serum-alkaline-phosphatase isoenzymes by polyacrylamide gel electrophoresis". The Lancet. 294 (7629): 1029–1031. doi:10.1016/s0140-6736(69)90640-0Immunoprecipitation (4,346 words) [view diff] exact match in snippet view article find links to article
done in a variety of ways: SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) followed by gel staining. SDS-PAGE followed by: gel stainingNPM1 (4,897 words) [view diff] exact match in snippet view article find links to article
liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis". Proceedings of the National Academy of Sciences of the UnitedTaphrina caerulescens (2,152 words) [view diff] exact match in snippet view article find links to article
Mycotaxon. 38: 315–30. Snider, R. D.; Kramer, C. L. (1974). "Polyacrylamide gel electrophoresis and numerical taxonomy of Taphrina caerulescens and TaphrinaGenomics (7,518 words) [view diff] exact match in snippet view article find links to article
fractionated by electrophoresis on a polyacrylamide gel (called polyacrylamide gel electrophoresis) and visualised using autoradiography. The procedure couldCD36 (5,216 words) [view diff] exact match in snippet view article find links to article
apparent molecular weight of 88,000 Da as determined by SDS polyacrylamide gel electrophoresis. Using Kyte-Doolittle analysis, the amino acid sequence ofVirology (7,208 words) [view diff] exact match in snippet view article find links to article
Polyacrylamide gel electrophoresis of rotavirus proteins stained with Coomassie blueGroEL (5,438 words) [view diff] exact match in snippet view article find links to article
human colon carcinoma proteins separated by two-dimensional polyacrylamide gel electrophoresis". Electrophoresis. 11 (10): 883–91. doi:10.1002/elps.1150111019Benjamin Weiss (scientist) (3,164 words) [view diff] exact match in snippet view article
3',5'‑monophosphate phosphodiesterase in rat cerebellum by polyacrylamide gel electrophoresis. Biochim. Biophys. Acta 284:220‑226, 1972. PMID 4342220 UzunovVenomics (3,657 words) [view diff] exact match in snippet view article find links to article
N-terminal end. Furthermore, SDS‐PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis) can be performed on the isolated proteins from the RP-HPLCTaylorella equigenitalis (4,118 words) [view diff] exact match in snippet view article find links to article
lipopolysaccharides by performing Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. Lipopolysaccharides typicallyOrientia tsutsugamushi (8,133 words) [view diff] exact match in snippet view article find links to article
composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting". Infection and Immunity. 48 (3): 671–675DNA nanotechnology (9,648 words) [view diff] case mismatch in snippet view article find links to article
2001). "Purification of Oligonucleotides Using Denaturing Polyacrylamide Gel Electrophoresis". Current Protocols in Molecular Biology. 42: Unit2.12. doi:10DNA-encoded chemical library (4,740 words) [view diff] exact match in snippet view article find links to article
helper oligonucleotide in P3. (b) The structure is purified by polyacrylamide gel electrophoresis (PAGE), the P1 and P2 DNA is ligated and the P2 linker isAcid dissociation constant (11,513 words) [view diff] exact match in snippet view article find links to article
focusing, which is used for separation of proteins by 2-D gel polyacrylamide gel electrophoresis. Buffer solutions also play a key role in analytical chemistrySodium dodecyl sulfate (3,064 words) [view diff] exact match in snippet view article find links to article
2017-02-16. The acronym expands to "sodium dodecyl sulfate-polyacrylamide gel electrophoresis." Janson LW, Tischler M (2012). The big picture: medical biochemistryRibose-seq (4,607 words) [view diff] case mismatch in snippet view article find links to article
introduced, and the ssDNA circles are amplified further. Polyacrylamide Gel Electrophoresis (PAGE) is performed to confirm the presence of the ribose-seqInsulin (13,791 words) [view diff] exact match in snippet view article find links to article
antibodies. When total translated protein was run on an SDS-polyacrylamide gel electrophoresis and sucrose gradient, peaks corresponding to insulin and proinsulinOligonucleotide synthesis (9,462 words) [view diff] exact match in snippet view article find links to article
truncation products, the oligonucleotides can be purified via polyacrylamide gel electrophoresis or anion-exchange HPLC followed by desalting. (2) The second