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Longer titles found: Recombinase-mediated cassette exchange (view), Recombinase polymerase amplification (view), Tre recombinase (view), Cre recombinase (view), Site-specific recombinase technology (view), Hin recombinase (view)

searching for Recombinase 91 found (175 total)

alternate case: recombinase

V(D)J recombination (2,860 words) [view diff] exact match in snippet view article

majority of T cells. The process of V(D)J recombination is mediated by VDJ recombinase, which is a diverse collection of enzymes. The key enzymes involved are

RAD51 (5,564 words) [view diff] exact match in snippet view article find links to article

RadB and RadC recombinase paralogs are found in many organisms belonging to Euryarchaeota while a broader diversity of related recombinase paralogs seem

Ortervirales (362 words) [view diff] exact match in snippet view article find links to article

(retroviral aspartyl protease) and an integrase belonging to the DDE recombinase superfamily (see Recombination-activating gene [structure]). They also

RAG2 (1,315 words) [view diff] exact match in snippet view article find links to article

human chromosome 11. Together with RAG1 protein, RAG2 forms a V(D)J recombinase, a protein complex required for the process of V(D)J recombination during

Conditional gene knockout (1,292 words) [view diff] exact match in snippet view article find links to article

commonly used technique is the Cre-lox recombination system. The Cre recombinase enzyme specifically recognizes two lox (loci of recombination) sites

P1 phage (2,000 words) [view diff] exact match in snippet view article find links to article

carry relatively large fragments of DNA. P1 encodes a site-specific recombinase, Cre, that is widely used to carry out cell-specific or time-specific

Susan Dymecki (2,397 words) [view diff] exact match in snippet view article find links to article

that enable transgenic expression of FRT recombination sites and a Flp recombinase in non-human mammals. Her technology has aided many researchers in achieving

Homologous recombination (12,105 words) [view diff] exact match in snippet view article find links to article

proteins of the RecA recombinase family of proteins are thought to be descended from a common ancestral recombinase. The RecA recombinase family contains RecA

Bacterial recombination (1,283 words) [view diff] exact match in snippet view article find links to article

Recombination in bacteria is ordinarily catalyzed by a RecA type of recombinase. These recombinases promote repair of DNA damages by homologous recombination.

Recombination signal sequences (663 words) [view diff] exact match in snippet view article find links to article

intervening DNA and creating a double-stranded break that is repaired by VDJ recombinase enzymes. This process is repeated for the joining of V to DJ. In light-chain

YqaJ protein domain (342 words) [view diff] exact match in snippet view article find links to article

5'-phosphorylated DNA ends. It thus forms part of the two-component SynExo viral recombinase functional unit. The function of this protein domain is to digest DNA

Spermatogenesis (3,731 words) [view diff] exact match in snippet view article find links to article

body temperature in most of mammals, as β-polimerase and spermatogenic recombinase need a specific optimal temperature. Consequently, the testes are located

Mosaic (genetics) (2,653 words) [view diff] exact match in snippet view article

flexible. The flip recombinase (or FLP) is a gene from the commonly studied yeast Saccharomyces cerevisiae that recognizes "flip recombinase target" (FRT)

Boris Zemelman (496 words) [view diff] exact match in snippet view article find links to article

dissertation on purification and characterization of a novel mammalian recombinase under professor I. Robert Lehman. He is now an assistant professor of

Stem cell theory of aging (2,579 words) [view diff] exact match in snippet view article find links to article

flip recombinase (Flp)-mediated recombination is used to combine a ubiquitously active tubulin promoter followed by an FRT (flip recombinase target)

Reverse genetics (2,635 words) [view diff] exact match in snippet view article find links to article

'knocking in' recombinase sites (such as lox or frt sites) that will cause a deletion at the gene of interest when a specific recombinase (such as CRE

Leishmania infantum (577 words) [view diff] exact match in snippet view article find links to article

function in homologous recombination and its interaction with the LiRAD51 recombinase. Aoun, K.; Bouratbine, A. (2014). "Cutaneous Leishmaniasis in North Africa:

Protein–protein interaction screening (726 words) [view diff] exact match in snippet view article find links to article

of proteins. Combining it with other new techniques, dual expression recombinase based (DERB) methods can enable the screening of protein–protein interactions

List of MeSH codes (D12.776.260) (972 words) [view diff] no match in snippet view article

The following is a partial list of the "D" codes for Medical Subject Headings (MeSH), as defined by the United States National Library of Medicine (NLM)

Protein–protein interaction screening (726 words) [view diff] exact match in snippet view article find links to article

of proteins. Combining it with other new techniques, dual expression recombinase based (DERB) methods can enable the screening of protein–protein interactions

Puromycin (829 words) [view diff] exact match in snippet view article find links to article

"Puromycin- and methotrexate-resistance cassettes and optimized Cre-recombinase expression plasmids for use in yeast". Yeast. 32 (5): 423–38. doi:10

Selectable marker (739 words) [view diff] exact match in snippet view article find links to article

instead relying on co-transformation, homologous recombination, and recombinase-mediated excision. Genetic marker Marker gene Biomarker "positive selection"

RecBCD (1,842 words) [view diff] exact match in snippet view article find links to article

converts the RecBCD enzyme of Escherichia coli into a Chi-independent recombinase by inactivation of the RecD subunit". Proc Natl Acad Sci U S A. 92 (14):

RI-1 (chemical) (280 words) [view diff] exact match in snippet view article

protein-protein interface that is essential for filament formation and recombinase activity. Targeted drugs to RAD51 has the prospect to be a new generation

Library (biology) (1,204 words) [view diff] exact match in snippet view article

A Cre-Lox system using loxP sites and the in vivo expression of the recombinase enzyme can also be used instead. These are examples of in vivo excision

RAD52 (3,013 words) [view diff] exact match in snippet view article find links to article

"Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form". Molecular Cell. 10 (2): 359–71. doi:10

Group II intron (1,854 words) [view diff] exact match in snippet view article find links to article

A, Boutard M, Salanoubat M, Tolonen AC (December 2019). "A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia". mSphere. 4

MYH10 (2,423 words) [view diff] exact match in snippet view article find links to article

knockout of NM-IIB using the alpha-myosin heavy chain promoter-driven cre-recombinase develop enlarged cardiomyocytes, consistent with the defects previously

PALB2 (1,567 words) [view diff] exact match in snippet view article find links to article

BRCA2. PALB2 binds the single strand DNA and directly interacts with the recombinase RAD51 to stimulate strand invasion, a vital step of homologous recombination

Anton Berns (723 words) [view diff] exact match in snippet view article find links to article

Both transgenesis and somatic gene transfer are employed to express Cre recombinase and other genes or shRNAs in a regulatable fashion. They have managed

Glomus (fungus) (989 words) [view diff] exact match in snippet view article

known function is in meiosis, including Dmc1 that is a meiosis-specific recombinase. Since meiosis is considered to be a hallmark of sexual reproduction

RAG1 (948 words) [view diff] exact match in snippet view article find links to article

endodeoxyribonuclease activity Cellular component nucleoplasm nucleus DNA recombinase complex endodeoxyribonuclease complex Biological process positive regulation

Tc1/mariner (1,828 words) [view diff] exact match in snippet view article find links to article

DDE (sometimes DDD) amino acids that are found in many transposase and recombinase enzymes. In addition, there is a region that is highly enriched in glycine

Regine Kahmann (1,171 words) [view diff] exact match in snippet view article find links to article

stimulated by the bacterial host's FIS protein in addition to the actual recombinase, the elucidation of the stimulation mechanism is internationally considered

Cytochrome c (3,790 words) [view diff] exact match in snippet view article find links to article

(October 2013). "An aptamer-based bio-barcode assay with isothermal recombinase polymerase amplification for cytochrome-c detection and anti-cancer drug

XRCC3 (1,745 words) [view diff] exact match in snippet view article find links to article

maintenance via recombination t-circle formation DNA repair meiotic DNA recombinase assembly interstrand cross-link repair double-strand break repair via

RAD51L1 (1,051 words) [view diff] exact match in snippet view article find links to article

transition of mitotic cell cycle mitotic recombination DNA repair meiotic DNA recombinase assembly double-strand break repair via homologous recombination Sources:Amigo

RAD54B (815 words) [view diff] exact match in snippet view article find links to article

PMID 12477932. Sehorn MG, Sigurdsson S, Bussen W, et al. (2004). "Human meiotic recombinase Dmc1 promotes ATP-dependent homologous DNA strand exchange". Nature.

Mutation (13,965 words) [view diff] exact match in snippet view article find links to article

expression seen during stages of development in model organisms. DNA Recombinase systems like Cre-Lox recombination used in association with promoters

GT198 (2,345 words) [view diff] exact match in snippet view article find links to article

stimulate Rad51-induced DNA strand exchange. GT198 may act similarly to DNA recombinase, an activity present in Rad51 homologs. GT198 forms heterodimer with

Mary Hatten (639 words) [view diff] exact match in snippet view article find links to article

Cummins, A., Hatten, M.E., Heintz, N., Gerfen, C.R. (2007). Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs

Non-homologous end joining (3,679 words) [view diff] exact match in snippet view article find links to article

Baltimore D (April 1988). "Stable expression of immunoglobulin gene V(D)J recombinase activity by gene transfer into 3T3 fibroblasts". Cell. 53 (1): 107–15

Severe combined immunodeficiency (3,231 words) [view diff] exact match in snippet view article find links to article

malfunction. Omenn syndrome The manufacture of immunoglobulins requires recombinase enzymes derived from the recombination activating genes RAG-1 and RAG-2

Deinococcus deserti (1,273 words) [view diff] exact match in snippet view article find links to article

using D. radiodurans as model. In cells subjected to irradiation, DNA recombinase, RecA, was the first protein that was found strongly induced. RecA is

NASBA (molecular biology) (1,586 words) [view diff] exact match in snippet view article

"Point-of-care diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method". The Journal of General Virology. 99

Protein-fragment complementation assay (1,265 words) [view diff] exact match in snippet view article find links to article

Deinococcus radiodurans LacZ (beta-galactosidase) Luciferase, including ReBiL (recombinase enhanced bimolecular luciferase) and Gaussia princeps luciferase. Commercial

XRCC2 (1,849 words) [view diff] exact match in snippet view article find links to article

response to gamma radiation DNA repair response to X-ray meiotic DNA recombinase assembly double-strand break repair via homologous recombination meiosis

RAD51L3 (1,335 words) [view diff] exact match in snippet view article find links to article

stimulus mitotic recombination telomere maintenance DNA repair meiotic DNA recombinase assembly double-strand break repair via homologous recombination Sources:Amigo

MARCM (1,436 words) [view diff] exact match in snippet view article find links to article

usually with a gene or mutation of interest distal to it. Finally, FLP recombinase is driven by an inducible promoter such as heat shock. When FLP transcription

Bacillus phage (650 words) [view diff] exact match in snippet view article find links to article

tail proteins and other structural proteins, holin, and a site-specific recombinase. One group of gene that is highly variable between Bacillus phage are

Simon Boulton (1,928 words) [view diff] exact match in snippet view article find links to article

achievements is the discovery that the gene RTEL1 serves as an anti-recombinase that affects genome stability and cancer and counteracts toxic recombination

Garnett H. Kelsoe (616 words) [view diff] exact match in snippet view article find links to article

Medicine. 187(6): pgs. 885-895 Han S, Dillon SR... Kelsoe G (1997). V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science. 278(5336):

Bacterial morphological plasticity (3,629 words) [view diff] exact match in snippet view article find links to article

different external cues (that induce mutations), the major bacterial recombinase (RecA) binds to this DNA regions and is activated by the presence of

Nav1.8 (3,618 words) [view diff] exact match in snippet view article find links to article

p11-null nociceptive sensory neurons in mice, created using the Cre-loxP recombinase system, show a decrease in Nav1.8 expression at the plasma membrane.

Norbert Perrimon (1,411 words) [view diff] exact match in snippet view article find links to article

Chou, T. B.; Perrimon, N. (1992-07-01). "Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila". Genetics. 131 (3):

DNA binding site (2,168 words) [view diff] exact match in snippet view article find links to article

the targets of other proteins, like restriction enzymes, site-specific recombinases (see site-specific recombination) and methyltransferases. DNA binding

Lumír Krejčí (1,021 words) [view diff] exact match in snippet view article find links to article

according to Google Scholar Sung P, Krejci L, Van Komen S, Sehorn MG. Rad51 recombinase and recombination mediators. Journal of Biological Chemistry. 2003 Oct

MecA (2,473 words) [view diff] exact match in snippet view article find links to article

contains mecA and two recombinase genes, ccrA and ccrB. Proper insertion of the mecA complex into the host genome requires the recombinases. Researchers have

Ebola virus disease treatment research (3,518 words) [view diff] exact match in snippet view article find links to article

diagnostic tests are under trial: Diagnostics-in-a-Suitcase, based on recombinase polymerase amplification (RPA). The new equipment, about the size of

Myc (6,709 words) [view diff] exact match in snippet view article find links to article

movements, etc.). The study by John M. Sedivy and others used Cre-Loxp -recombinase to knockout one copy of Myc and this resulted in a "Haplo-insufficient"

Human mitochondrial genetics (4,057 words) [view diff] exact match in snippet view article find links to article

mutations that may occur due to free radicals. It is believed that a DNA recombinase found in mammalian cells is also involved in a repairing recombination

Protein splicing (3,821 words) [view diff] exact match in snippet view article find links to article

inteins within DnaB (bacterial replicative helicase), RecA (bacterial DNA recombinase), and SufB (FeS cluster assembly protein). There is remarkable variety

Limosilactobacillus pontis (1,981 words) [view diff] exact match in snippet view article find links to article

for elongation factor tu, type strain DSM 8474T. Partial recA gene for recombinase A, strain LMG 14187T. Complete rpoA gene for RNA polymerase alpha subunit

Isogenic human disease models (2,046 words) [view diff] exact match in snippet view article find links to article

transgenes at a single pre-defined genomic locus (integration via a FLP recombinase site). This is not a technique for modifying an endogenous locus. Transgenes

Gottfriedia (914 words) [view diff] exact match in snippet view article find links to article

class I SAM-dependent RNA methyltransferase, site-specific tyrosine recombinase XerD, 3-isopropylmalate dehydrogenase, 5-oxoprolinase subunit PxpB, MATE

Fear (12,321 words) [view diff] exact match in snippet view article find links to article

glucocorticoid receptors were inhibited using lentiviral vectors containing Cre-recombinase injected into mice. Results showed that disruption of the glucocorticoid

Lambda phage (5,929 words) [view diff] exact match in snippet view article find links to article

vector for the cloning of recombinant DNA; the use of its site-specific recombinase (int) for the shuffling of cloned DNAs by the gateway method; and the

Patricia Janak (1,268 words) [view diff] exact match in snippet view article find links to article

EE Steinberg, SY Lee, TJ Davidson, KA Zalocusky, M Brodsky, et al., Recombinase-driver rat lines: tools, techniques, and optogenetic application to dopamine-mediated

Helicoverpa zea nudivirus 2 (4,466 words) [view diff] exact match in snippet view article find links to article

of Hz2V008 has homology with the catalytic domain for integrase and recombinase, which has DNA breaking and rejoining activity. This region of HsNV-2's

RAD51C (2,939 words) [view diff] exact match in snippet view article find links to article

cycle mitotic recombination DNA repair double-strand break repair via homologous recombination meiotic DNA recombinase assembly Sources:Amigo / QuickGO

DNA adenine methyltransferase identification (2,212 words) [view diff] exact match in snippet view article find links to article

can be removed when combined with tissue specific expression of FLP recombinase. Upon removal of the cassette, the Dam-fusion is expressed at low levels

Human genetic enhancement (10,464 words) [view diff] exact match in snippet view article find links to article

(June 2020). "Successful Preclinical Development of Gene Therapy for Recombinase-Activating Gene-1-Deficient SCID". Molecular Therapy. Methods & Clinical

Balancer chromosome (2,112 words) [view diff] exact match in snippet view article find links to article

and keep them in a constant line. They achieved this by using the FLP recombinase gene, isolated from yeast, which causes large chromosomal inversions

Evolution of sexual reproduction (9,444 words) [view diff] exact match in snippet view article find links to article

transformation in bacteria and DNA transfer in archaea. For example, recA recombinase, that catalyses the key functions of DNA homology search and strand exchange

George Yancopoulos (2,391 words) [view diff] exact match in snippet view article find links to article

segments recombine in pre-B cells: evidence that B and T cells use a common recombinase". Cell. 44 (2): 251–9. doi:10.1016/0092-8674(86)90759-2. PMID 3484682

Protein ZGRF1 (1,714 words) [view diff] exact match in snippet view article find links to article

camptothecin. Mechanistically, ZGRF1 physically interacts with the RAD51 recombinase and stimulates strand exchange by RAD51-RAD54. ZGRF1 shares homology

RNA interference (15,445 words) [view diff] exact match in snippet view article find links to article

S2CID 18460980. Tiscornia G, Tergaonkar V, Galimi F, Verma IM (May 2004). "CRE recombinase-inducible RNA interference mediated by lentiviral vectors". Proceedings

STIL (2,251 words) [view diff] exact match in snippet view article find links to article

(1991). "Disruption of the human SCL locus by "illegitimate" V-(D)-J recombinase activity". Science. 250 (4986): 1426–9. doi:10.1126/science.2255914.

Chester L. Sutula (1,108 words) [view diff] exact match in snippet view article find links to article

offers more than 200 tests that use several technologies, such as ELISA, Recombinase Polymerase Amplification (RPA), Polymerase Chain Reaction (PCR), nucleic

Sanofi Biogenius Canada (1,352 words) [view diff] exact match in snippet view article find links to article

Burnaby, BC "Microfluidic Point-of-Care Device for Diagnosis of HIV via Recombinase Polymerase Amplification." A novel method of HIV detection in developing

Methods to investigate protein–protein interactions (4,494 words) [view diff] exact match in snippet view article find links to article

PMID 11983170. Lu JP, Beatty LK, Pinthus JH (2008). "Dual expression recombinase based (DERB) single vector system for high throughput screening and verification

CD4+ T cells and antitumor immunity (4,000 words) [view diff] exact match in snippet view article find links to article

immunosurveillance theory. In 2001, it was shown that mice deficient in RAG-2 (Recombinase Activator Gene 2) were far less capable of preventing MCA induced tumours

Glossary of cellular and molecular biology (0–L) (24,805 words) [view diff] exact match in snippet view article

consisting of a gene cassette containing the gene for a site-specific recombinase, integrase-specific recognition sites, and a promoter that governs the

Phasevarion (1,582 words) [view diff] exact match in snippet view article find links to article

variable hsd genes present in the locus. Recombination is catalyzed by a recombinase that is associated with the type I locus. These six methyltransferase

Sandro Santagata (1,492 words) [view diff] exact match in snippet view article find links to article

discovered that a partial loss of function in the RAG components of the V(D)J recombinase causes Omenn syndrome, a severe immunodeficiency disorder. While at Mount

Transplastomic plant (3,091 words) [view diff] exact match in snippet view article find links to article

been implemented is the Cre/lox system, where the nuclear encoded Cre recombinase can be placed under control of an inducible promoter to remove the antibiotic

Carolyn I. Rodriguez (2,679 words) [view diff] exact match in snippet view article find links to article

precerebellar neurons, Rodriguez developed a genetic tool, using flip-recombinase, that allowed for irreversible fluorescent tagging of descendent cells

Cell-penetrating peptide (7,214 words) [view diff] exact match in snippet view article find links to article

by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was

No-SCAR genome editing (5,650 words) [view diff] exact match in snippet view article find links to article

3’ overhangs on the linear DNA. Subsequently, λ beta, a member of a recombinase family, binds to the 3’ overhangs and mediates annealing to the complementary

Minimal genome (5,898 words) [view diff] exact match in snippet view article find links to article

comparison 2002 S. cerevisiae Systematic gene deletion 2002 S. aureus Antisense RNA 2002 E. coli Red recombinase excision 2002 E. coli Cre/loxP excision

Double-strand break repair model (4,127 words) [view diff] exact match in snippet view article find links to article

DSB are processed into ssDNA with 3’ extensions, which allows RAD51 recombinase (eukaryotic homologue of prokaryotic RecA) to bind to it to form a nucleoprotein