Find link

language:

Find link is a tool written by Edward Betts.

searching for Restriction enzyme 80 found (262 total)

alternate case: restriction enzyme

Palindromic sequence (823 words) [view diff] exact match in snippet view article find links to article

enzymes) recognize specific palindromic sequences and cut them. The restriction enzyme EcoR1 recognizes the following palindromic sequence: 5'- G A A T T

Representation oligonucleotide microarray analysis (416 words) [view diff] exact match in snippet view article find links to article

has the advantage of reducing the complexity of a genome with a restriction enzyme which highly increases the efficiency of genomic fragment hybridization

Ligation-independent cloning (156 words) [view diff] exact match in snippet view article find links to article

The technique was developed in the early 1990s as an alternative to restriction enzyme/ligase cloning. This allows genes to be cloned without the requirement

Optical mapping (2,102 words) [view diff] exact match in snippet view article find links to article

molecules of DNA, called "optical maps". By mapping the location of restriction enzyme sites along the unknown DNA of an organism, the spectrum of resulting

Cleaved amplified polymorphic sequence (158 words) [view diff] exact match in snippet view article find links to article

the altered restriction site, and the products digested with the restriction enzyme. When fractionated by agarose or polyacrylamide gel electrophoresis

Gateway Technology (1,332 words) [view diff] exact match in snippet view article find links to article

more time for initial set-up, and is more expensive than traditional restriction enzyme and ligase-based cloning methods, but it saves time, and offers simpler

Bacillus amyloliquefaciens (1,214 words) [view diff] exact match in snippet view article find links to article

of bacterium in the genus Bacillus that is the source of the BamHI restriction enzyme. It also synthesizes a natural antibiotic protein barnase, a widely

Combined bisulfite restriction analysis (1,299 words) [view diff] exact match in snippet view article find links to article

to the methylation dependent retention or loss of CpG-containing restriction enzyme sites, such as those for TaqI (TCGA) and BstUI (CGCG), depending on

Brevinema andersonii (874 words) [view diff] exact match in snippet view article find links to article

laboratory mice and Syrian hamsters.B. andersonii is readily identified by restriction enzyme analysis, and SDS-PAGE, or fatty acid composition data. Another identifier

Gibson assembly (689 words) [view diff] exact match in snippet view article find links to article

DNA assembly method has many advantages compared to conventional restriction enzyme/ligation cloning of recombinant DNA. For example, No restriction digest

Restriction endonuclease BsobI/AvaI (102 words) [view diff] exact match in snippet view article find links to article

van der Woerd MJ, Pelletier JJ, Xu S, Friedman AM (February 2001). "Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences

CAG promoter (444 words) [view diff] exact match in snippet view article find links to article

proximal region of the second exon was disrupted by digesting with NcoI restriction enzyme and replacing the site with a HindIII linker. Although the whole construct

Disciotis (215 words) [view diff] exact match in snippet view article find links to article

Morchella, Verpa, and Disciotis (Pezizales: Morchellaceae) based on restriction enzyme analysis of the 28S ribosomal RNA gene. Experimental Mycology 19 (3):

Morchellaceae (393 words) [view diff] exact match in snippet view article find links to article

Morchella, Verpa, and Disciotis (Pezizales: Morchellaceae) based on restriction enzyme analysis of the 28S ribosomal RNA gene". Experimental Mycology. 19

Coprinopsis cinerea (1,455 words) [view diff] exact match in snippet view article find links to article

be used, likely with low transformation efficiency. Earlier, REMI (restriction enzyme-mediated integration) could be used to insert exogenous DNA into the

B3 domain (726 words) [view diff] exact match in snippet view article find links to article

G, Huber R, Siksnys V (2005). "Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific

Nicking enzyme (396 words) [view diff] exact match in snippet view article find links to article

Shank DD (Jan 1992). "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system". Proc. Natl. Acad. Sci. U.S.A. 89 (1): 392–6

EMBOSS (324 words) [view diff] exact match in snippet view article find links to article

Nucleic repeats Nucleic acid repeat detection Nucleic restriction Restriction enzyme sites in nucleotide sequences Nucleic RNA folding RNA folding methods

Epigenome (1,171 words) [view diff] case mismatch in snippet view article find links to article

Immunoprecipitation Sequencing (MeDIP-Seq), and Methylation-sensitive Restriction Enzyme Sequencing (MRE-Seq) identify DNA methylation across portions of the

Michael McClelland (academic) (867 words) [view diff] case mismatch in snippet view article

and Restriction Enzyme Recognition Sequences. IRL Press. 1989. TRNA, Cloned Human DNA and E. Coli Sequences, Histone Genes and Restriction Enzyme Recognition

BLESS (1,978 words) [view diff] exact match in snippet view article find links to article

barcode sequence that labels the site of ligation as well as an XhoI restriction enzyme recognition site adjacent to the barcode. The hairpin loop of the

Fasciola gigantica (1,213 words) [view diff] exact match in snippet view article find links to article

differentiation of Fasciola hepatica and Fasciola gigantica using a simple PCR-restriction enzyme method". Experimental Parasitology. 124 (2): 209–213. doi:10.1016/j

Nannizzia fulva (90 words) [view diff] exact match in snippet view article find links to article

"Phylogeny of Nannizzia incurvata, N. gypsea, N. fulva and N. otae by restriction enzyme analysis of mitochondrial DNA". Mycopathologia. 112 (3): 173–177.

Haplogroup R2 (462 words) [view diff] exact match in snippet view article find links to article

(bp) reference sequence 323 Polymorphism position from 5' end 107 Restriction enzyme variant HphI RefSNP ID - Y-position 19294055 Primer forward 5'-3'

Nannizzia (235 words) [view diff] exact match in snippet view article find links to article

"Phylogeny of Nannizzia incurvata, N. gypsea, N. fulva and N. otae by restriction enzyme analysis of mitochondrial DNA". Mycopathologia. 112 (3): 173–177.

Protein engineering (7,479 words) [view diff] exact match in snippet view article find links to article

N-terminus. These hybrid gene fragments are separated using either restriction enzyme digestion or PCR with terminus primers via agarose gel electrophoresis

Computational epigenetics (1,085 words) [view diff] case mismatch in snippet view article find links to article

Methylation Database Pubmeth.Org "Official REBASE Homepage | the Restriction Enzyme Database | NEB". "DeepBlue Epigenomic Data Server". "MeInfoText: associated

Morchella elata (1,566 words) [view diff] exact match in snippet view article find links to article

Morchella, Verpa, andDisciotis (Pezizales: Morchellaceae) based on restriction enzyme analysis of the 28S ribosomal RNA gene". Experimental Mycology. 19

Epidermophyton floccosum (1,518 words) [view diff] exact match in snippet view article find links to article

distinguishes between 12 dermatophyte species based on their individual restriction enzyme profiles, including one for E. floccosum. A real time PCR protocol

TOPO cloning (568 words) [view diff] exact match in snippet view article find links to article

adenine-overhangs. The target vector is linearized and cut with a blunt-end restriction enzyme. This vector is then tailed with dideoxythymidine triphosphate (ddTTP)

Population genomics (2,149 words) [view diff] exact match in snippet view article find links to article

genetic variability at multiple loci included gel electrophoresis and restriction enzyme mapping. Previously genomics was restricted to only the study of a

Nannizzia incurvata (164 words) [view diff] exact match in snippet view article find links to article

"Phylogeny of Nannizzia incurvata, N. gypsea, N. fulva and N. otae by restriction enzyme analysis of mitochondrial DNA". Mycopathologia. 112 (3): 173–177.

Overlap extension polymerase chain reaction (1,051 words) [view diff] exact match in snippet view article find links to article

as Gibson assembly, which overcome the limitations of traditional restriction enzyme digestion and ligation cloning methods. Assembly of custom DNA sequences

HLA-DQA2 (1,057 words) [view diff] exact match in snippet view article find links to article

"Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes". Proc. Natl. Acad

Addgene (756 words) [view diff] exact match in snippet view article find links to article

scientists design plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning. Molecular Cloning Protocols—Specific

Tapesia yallundae (665 words) [view diff] exact match in snippet view article find links to article

compact and mounded. Polymerase chain reaction (PCR) combined with restriction enzyme digestion of an amplified ribosomal DNA fragment, are now used to

V. Nagaraja (635 words) [view diff] exact match in snippet view article find links to article

DNA transaction enzymes, design and development of high fidelity restriction enzyme, new inhibitors for the generation of lead molecules as a step for

Site-specific DNA-methyltransferase (cytosine-N4-specific) (249 words) [view diff] case mismatch in snippet view article

PMID 6267988. Roberts RJ. "A complete listing of all of these enzymes". The Restriction Enzyme Database. Site-specific+DNA-methyltransferase+(cytosine-N4-specific)

Sulfolobus acidocaldarius (956 words) [view diff] exact match in snippet view article find links to article

rescues Sulfolobales from DNA damaging threats. The thermostable restriction enzyme SuaI is obtained from this organism. Brock, TD; Brock, KM; Belly,

Factor XII (3,274 words) [view diff] exact match in snippet view article find links to article

1987). "Factor XII gene alteration in Hageman trait detected by TaqI restriction enzyme". Blood. 69 (5): 1421–1424. doi:10.1182/blood.V69.5.1421.1421. PMID 2882793

Biotechnology in pharmaceutical manufacturing (1,536 words) [view diff] exact match in snippet view article find links to article

found in pituitary glands to complementary DNA. HaeIII, a type of restriction enzyme which acts at restriction sites "in the 3' noncoding region" and at

Ground squirrel hepatitis virus (1,704 words) [view diff] exact match in snippet view article find links to article

are similar to HBV and WHV, but the viruses differ in their EcoRI restriction enzyme recognition sites. Ground squirrel hepatitis virus is a spherical

Reverse genetics (2,635 words) [view diff] exact match in snippet view article find links to article

RNA polymerase I (Pol I) promoter and terminator sequence through restriction enzyme digestion. The cDNA and pol I sequence is then, in turn, surrounded

Entamoeba histolytica (3,056 words) [view diff] exact match in snippet view article find links to article

Rao DN, Bhattacharya S (December 2009). "Characterization of the restriction enzyme-like endonuclease encoded by the Entamoeba histolytica non-long terminal

Recombinant DNA (3,893 words) [view diff] exact match in snippet view article find links to article

DNA segments can be combined by using a variety of methods, such as restriction enzyme/ligase cloning or Gibson assembly.[citation needed] In standard cloning

HLA-DQ1 (1,480 words) [view diff] exact match in snippet view article find links to article

"Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes". Proc. Natl. Acad

Salvelinus neiva (187 words) [view diff] case mismatch in snippet view article find links to article

8 December 2023. Radchenko, O. A. (1 January 2003). "Comparative Restriction Enzyme Analysis of Mitochondrial DNA in Several Populations of Lake Resident

Maryland v. King (2,247 words) [view diff] exact match in snippet view article find links to article

The DNA from the cheek cells in the swab is replicated, is given a restriction enzyme digest, and is electrophoresed. Electrophoresis separates DNA segments

Natural competence (2,641 words) [view diff] exact match in snippet view article find links to article

3-Sex pili 4-Plasmid of foreign DNA from a dead cell 5-Bacterial cell restriction enzyme 6-Unwound foreign plasmid 7-DNA ligase I: A plasmid of foreign DNA

COLD-PCR (1,607 words) [view diff] exact match in snippet view article find links to article

(or absence thereof) of DNA for a specific mutation by a selected restriction enzyme that will not cleave the wildtype DNA. In a study using a mixture

Morchella (6,361 words) [view diff] exact match in snippet view article find links to article

Morchella, Verpa, andDisciotis (Pezizales: Morchellaceae) based on restriction enzyme analysis of the 28S ribosomal RNA gene". Experimental Mycology. 19

Mobile genetic elements (3,783 words) [view diff] exact match in snippet view article find links to article

vector plasmid. This vector contains the origin of replication sequence, the restriction enzyme cut site, lacZ gene, and an ampicillin resistance gene.

Joanna Wilson (915 words) [view diff] exact match in snippet view article find links to article

Y Wilson, Christopher M Somers. 2015. Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADS eq). Molecular Ecology Resources,

Rolling circle replication (2,920 words) [view diff] exact match in snippet view article find links to article

amplification or C2CA, where the RCA products are digested with a restriction enzyme and ligated into new circular templates using a restriction oligo

Genetically modified virus (3,843 words) [view diff] exact match in snippet view article find links to article

into a viral genome was published, when Paul Berg used the EcoRI restriction enzyme and DNA ligases to create the first ever recombinant DNA molecules

Stanley Falkow (4,181 words) [view diff] exact match in snippet view article find links to article

Waikiki beach meeting in 1972 between Herb Boyer (who provided the restriction enzyme EcoRI) and Stanley Cohen (who provided the plasmid) that led to a

Pelham Memorial High School (3,208 words) [view diff] exact match in snippet view article find links to article

techniques, such as DNA amplification via polymerase chain reaction, restriction enzyme analysis, and bacterial culture, identification, and transformation

Chromatin (5,949 words) [view diff] case mismatch in snippet view article find links to article

Randall H. (ed.), "Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility", Chromatin Remodeling, Methods in Molecular Biology

PTC tasting (1,442 words) [view diff] exact match in snippet view article find links to article

TAS2R38. The amplified fragment (amplicon) is incubated with the restriction enzyme HaeIII, comprising the SNP in their recognition sequence GGCC. HaeIII

Pulsed-field gel electrophoresis (2,652 words) [view diff] exact match in snippet view article find links to article

chamber. Loading the DNA sample: The DNA sample is digested with a restriction enzyme to generate a set of fragments of known size. Restriction enzymes

Mayan genetics (1,497 words) [view diff] exact match in snippet view article find links to article

and D.. Haplogroup A, (np) 16111 mutation of C to T and a Hae III Restriction enzyme site at np 633. mtDNA HincII morph-6 (site loss at np 13 259) and

DNA adenine methylase (1,844 words) [view diff] case mismatch in snippet view article find links to article

50.070181.001441. PMID 6267988. Roberts RJ, Macelis D (eds.). "The Restriction Enzyme Database". REBASE. Retrieved 22 Feb 2020. Aughey GN, Southall TD (January

Thomas J. Kelly (scientist) (1,237 words) [view diff] exact match in snippet view article

on March 5, 2013 Kelly, Thomas J.; Smith, Hamilton O. (1970). "A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition site"

Rhesus macaque (9,079 words) [view diff] exact match in snippet view article find links to article

rheusus monkeys (Macaca mulatta) as revealed by mitochondrial DNA restriction enzyme analysis". International Journal of Primatology. 14 (4): 587. doi:10

APOBEC3A (1,700 words) [view diff] exact match in snippet view article find links to article

"Methylcytosine and normal cytosine deamination by the foreign DNA restriction enzyme APOBEC3A". The Journal of Biological Chemistry. 287 (41): 34801–8

Ligation (molecular biology) (4,862 words) [view diff] exact match in snippet view article

The information on the minimum basepair required is available from restriction enzyme suppliers such as in the catalog of New England Biolabs. Incomplete

Arthur Riggs (geneticist) (2,448 words) [view diff] exact match in snippet view article

hypothesized that X chromosome inactivation might act in ways analogous to restriction enzyme complexes such as E. coli. He eventually published a theoretical paper

In vitro compartmentalization (2,195 words) [view diff] exact match in snippet view article find links to article

expressed methyltransferase, would be methylated and, thus, resistant to restriction enzyme digestion. By selecting for DNA sequences that survive the endonuclease

Raccoonpox virus (1,955 words) [view diff] exact match in snippet view article find links to article

of the genus. Differences between terminal region patterns of DNA restriction enzyme digests of different RCN isolates suggest that varied passaging has

Human variability (5,461 words) [view diff] exact match in snippet view article find links to article

human population studies based on mitochondrial DNA were performed by restriction enzyme analyses (RFLPs) and revealed differences between the four ethnic

Masai ostrich (2,497 words) [view diff] no match in snippet view article find links to article

blue-necked forms (S. c. australis and S. c. molybdyphanes). Comparative restriction-enzyme analysis of mitochondrial DNA (mtDNA) found the Masai ostrich, though

Histophilus somni (3,434 words) [view diff] exact match in snippet view article find links to article

differentiation between ovine and bovine strains of H. somni, including restriction enzyme analysis. Microscopy can be used for identification when bacteria

Artificial gene synthesis (7,073 words) [view diff] exact match in snippet view article find links to article

negatively affect protein folding or expression. By using the BsaI restriction enzyme that produces a 4 base pair overhang, up to 240 unique, non-palindromic

Zinc finger nuclease treatment of HIV (3,400 words) [view diff] exact match in snippet view article find links to article

restriction modification (R-M) systems. These bacteria secrete a restriction enzyme (REase) that recognizes and repetitively cleaves around palindromic

Whole genome bisulfite sequencing (2,517 words) [view diff] exact match in snippet view article find links to article

methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods". Genome Research. 23 (9): 1541–1553. doi:10.1101/gr

Glossary of cellular and molecular biology (0–L) (23,055 words) [view diff] exact match in snippet view article

synthetic DNA duplex containing the recognition sequence for a particular restriction enzyme. In molecular cloning, linkers are often deliberately included in

Zeda Rosenberg (2,427 words) [view diff] exact match in snippet view article find links to article

355-359 (1980). Sahagan, B.G., Rosenberg, Z.F., and Haseltine, W.A. Restriction enzyme analysis of the feline sarcoma virus provirus in murine fibroblasts

Magnetic tweezers (4,901 words) [view diff] exact match in snippet view article find links to article

more about DNA-binding proteins, receptor-ligand interactions, and restriction enzyme cleavage. A more recent application of magnetic tweezers is seen in

Nucleoid (17,198 words) [view diff] exact match in snippet view article find links to article

between genomic loci. Subsequently, the genome is digested with a restriction enzyme. In the next step, a DNA ligation is carried out under diluted DNA

Actinobacillus equuli (4,998 words) [view diff] exact match in snippet view article find links to article

from the oral cavity of healthy horses and comparison of isolates by restriction enzyme digestion and Pulsed-Field Gel Electrophoresis". Veterinary Microbiology